Delayed Encounter of Parental Genomes Can Lead to Aneuploidy in Saccharomyces cerevisiae
| Autor: | David Dulce, Alan M. Tartakoff, Elizabeth Landis |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Centromere Saccharomyces cerevisiae Fluorescent Antibody Technique Loss of Heterozygosity Aneuploidy Spindle Apparatus Investigations Biology Spindle pole body Karyogamy 03 medical and health sciences 0302 clinical medicine Genetics medicine Cell Nucleus Zygote Nuclear Proteins Chromosome medicine.disease biology.organism_classification 030104 developmental biology medicine.anatomical_structure Haplotypes Spindle Pole Bodies Chromosomes Fungal Genome Fungal Nucleus 030217 neurology & neurosurgery |
| Zdroj: | Genetics. 208:139-151 |
| ISSN: | 1943-2631 |
| Popis: | We have investigated an extreme deviation from the norm of genome unification that occurs during mating in the yeast, Saccharomyces cerevisiae. This deviation is encountered when yeast that carry a mutation of the spindle pole body protein, Kar1, are mated with wildtype cells. In this case, nuclear fusion is delayed and the genotypes of a fraction of zygotic progeny suggest that chromosomes have “transferred” between the parental nuclei in zygotes. This classic, yet bizarre, occurrence is routinely used to generate aneuploid (disomic) yeast. [kar1 × wt] zygotes, like [wt × wt] zygotes, initially have a single spindle pole body per nucleus. Unlike [wt × wt] zygotes, in [kar1 × wt] zygotes, the number of spindle pole bodies per nucleus then can increase before nuclear fusion. When such nuclei fuse, the spindle pole bodies do not coalesce efficiently, and subsets of spindle pole bodies and centromeres can enter buds. The genotypes of corresponding biparental progeny show evidence of extensive haplotype-biased chromosome loss, and can also include heterotypic chromosomal markers. They thus allow rationalization of chromosome “transfer” as being due to an unanticipated yet plausible mechanism. Perturbation of the unification of genomes likely contributes to infertility in other organisms. |
| Databáze: | OpenAIRE |
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