Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay
| Autor: | Ahmad Ali Shahid, Peter Wiegand, Muhammad Saqib Shahzad, Galina Kulstein, Hwan Young Lee, Mian Sahib Zar, Muhammad Israr, Sang Seob Lee, Kyoung Jin Shin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genotype Genotyping Techniques Population Single-nucleotide polymorphism Computational biology Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Bone and Bones Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Gene Frequency Genetics Humans SNP Multiplex 030216 legal & forensic medicine education Genotyping education.field_of_study DNA Degradation Necrotic Single-base extension DNA Fingerprinting SNP genotyping 030104 developmental biology |
| Zdroj: | Journal of Forensic Sciences. 63:1824-1829 |
| ISSN: | 0022-1198 |
| DOI: | 10.1111/1556-4029.13783 |
| Popis: | This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases. |
| Databáze: | OpenAIRE |
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