Establishment of an Enzyme-Linked Immuno-Sorbent Assay for Urinary Trypsin Inhibitor by Using a Monoclonal Antibody
| Autor: | G. Trefz, C. W. E. Justus, M. D. Kramer, B. Streit, W. Ebert |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Lung Diseases
Lung Neoplasms medicine.drug_class Immunoblotting Immunology Enzyme-Linked Immunosorbent Assay Urine urologic and male genital diseases Monoclonal antibody Alpha-Globulins medicine Humans Empyema Tuberculosis Pulmonary Glycoproteins Pharmacology chemistry.chemical_classification Lung Pancreatic Elastase biology Respiratory disease Antibodies Monoclonal bacterial infections and mycoses Trypsin medicine.disease Molecular biology female genital diseases and pregnancy complications medicine.anatomical_structure Enzyme chemistry Enzyme inhibitor alpha 1-Antitrypsin biology.protein Leukocyte Elastase Trypsin Inhibitors Clone (B-cell biology) Biomarkers medicine.drug |
| Zdroj: | Journal of Immunoassay. 12:347-369 |
| ISSN: | 0197-1522 |
| DOI: | 10.1080/01971529108055077 |
| Popis: | Monoclonal antibodies against inter-α-trypsin-inhibitor (ITI) were produced. One clone showing specificity for urinary trypsin inhibitor (UTI), a proteolytic fragment of ITI, which is excreted into urine, was selected for the establishment of an enzyme-linked immuno-sorbent assay (ELISA). The ELISA for the quantification of UTI was shown to work reproducibly in the range between 0.5 and 10 ng UTI/ml urine. Urines of several patients suffering from different lung diseases were screened for UTI using the established ELISA. Highest UTI levels were found in the urine of patients with lung empyema. A more moderate increase was observed in patients suffering from lung tuberculosis and from secondary and primary lung tumors. |
| Databáze: | OpenAIRE |
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