Organic/inorganic hydrogels by simultaneous self-assembly and mineralization of aromatic short-peptides
| Autor: | Gloria Belén Ramírez-Rodríguez, Jose Ignacio Garcia, Juan Manuel Cuerva Carvajal, José Manuel Delgado-López, Modesto T Lopez-Lopez, Luis Alvarez de Cienfuegos, Mari C. Mañas - Torres, Belén Parra-Torrejón |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Ministerio de Economía y Competitividad (España), [Manas-Torres, Mari C.] Univ Granada UGR, Fac Ciencias, Dept Quim Organ, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada 18071, Spain, [Cuerva, Juan M.] Univ Granada UGR, Fac Ciencias, Dept Quim Organ, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada 18071, Spain, [Alvarez de Cienfuegos, Luis] Univ Granada UGR, Fac Ciencias, Dept Quim Organ, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada 18071, Spain, [Ramirez-Rodriguez, Gloria B.] Fac Ciencias, Dept Quim Inorgan, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada, Spain, [Parra-Torrejon, Belen] Fac Ciencias, Dept Quim Inorgan, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada, Spain, [Delgado-Lopez, Jose M.] Fac Ciencias, Dept Quim Inorgan, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada, Spain, [Garcia-Peiro, Jose, I] Univ Zaragoza, CSIC, Inst Nanociencia & Mat Aragon, Zaragoza 50009, Spain, [Garcia-Peiro, Jose, I] Univ Zaragoza, Dept Ingn Quim & Tecnol Medioambiental IQTMA, Zaragoza 50018, Spain, [Lopez-Lopez, Modesto T.] UGR, Dept Fis Aplicada, Fac Ciencias, Granada, Spain, [Lopez-Lopez, Modesto T.] Inst Invest Biosanit Ibs GRANADA, Granada, Spain, [Alvarez de Cienfuegos, Luis] Inst Invest Biosanit Ibs GRANADA, Granada, Spain, FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades (Spain), FSE 'El FSE invierte en tu futuro', Spain, Junta de Andalucia |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | This study was supported by projects RTI-2018-095794-A-C22, RYC-2016-21042 and project PID2020-118498GB-I00 funded by MCIN/AEI/10.13039/501100011033 and projects P18-FR-3533 and P18-TP-0969 by FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades (Spain). Thanks go to the CIC personnel of the University of Granada for technical assistance. MCMT acknowledges grant PRE2018-083773 funded by MCIN/AEI/10.13039/501100011033 and FSE "El FSE invierte en tu futuro", Spain. GBRR also acknowledges Junta de Andalucia for her postdoctoral contract within the PAIDI 2020 program ( DOC_01383). Self-assembled peptides and proteins have turned out to be excellent templates for the growth of inorganic minerals and can be used to emulate natural biomineralization processes. Doing this, researchers have developed complex sophisticated materials with properties, in some cases, similar to those found in nature. Of special interest is the development of scaffolds able to guide bone regeneration. The bone tissue comprises an organic matrix composed of aligned collagen fibers containing nanoapatite crystals oriented along the fiber direction. During bone mineralization, both processes, the self-assembly of collagen fibrils and mineralization occur simultaneously. Collagen fibers are able to control calcium phosphate nucleation and subsequent apatite crystal growth at a very limited range of collagen density and ionic concentration. In this study, we reproduced the simultaneity of both processes using an artificial peptide fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) that has the ability to self-assemble in water after the addition of Ca2+ ions. Therefore, the peptide self-assembly process and the mineralization of apatite are Ca-demanding processes and occur simultaneously. The role of peptide and ionic concentrations has been investigated affording organic/inorganic hybrid hydrogels with different degrees of homogeneity and mineralization. Interestingly, at very low Ca2+ concentrations, we found that apatite nanocrystals are integrated into Fmoc-FF fibrils and oriented as in biologically mineralized collagen fibrils, the basic building blocks of bone. FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades (Spain) P18-FR-3533 P18-TP-0969 FSE "El FSE invierte en tu futuro", Spain Junta de Andalucia DOC_01383; RTI-2018-095794-A-C22; RYC-2016-21042; PID2020-118498GB-I00; MCIN/AEI/10.13039/501100011033; PRE2018-083773 |
| Databáze: | OpenAIRE |
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