cDNA Cloning and Characterization of a Human Sperm Antigen (SPAG6) with Homology to the Product of the Chlamydomonas PF16 Locus
| Autor: | Marianthi Kiriakidou, Lorna I. Neilson, Peter G. Van Deerlin, Karen K. Yamamoto, Deborah A. Driscoll, Cynthia K. French, Patrick A. Schneider, Shawn Millinder, Maria Pellegrini, Jerome F. Strauss |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Axoneme Databases Factual Molecular Sequence Data Gene Expression Chlamydomonas reinhardtii Locus (genetics) Antibodies Gene mapping Complementary DNA Testis Genetics Animals Humans Tissue Distribution Amino Acid Sequence Antigens Cloning Molecular Peptide sequence Conserved Sequence In Situ Hybridization Fluorescence Infertility Male Phylogeny Gene Library Sequence Homology Amino Acid biology Immune Sera Algal Proteins Chlamydomonas DNA biology.organism_classification Immunohistochemistry Spermatozoa Molecular biology Recombinant Proteins Clone Cells Armadillo repeats Microtubule Proteins RNA |
| Zdroj: | Genomics. 60:272-280 |
| ISSN: | 0888-7543 |
| Popis: | Serum from an infertile male with high-titer anti-sperm antibodies was used to identify a novel human sperm antigen by screening of a testis expression library. The clone, initially designated Repro-SA-1 (HUGO-approved symbol SPAG6), was found to encode a sequence highly enriched in testis. The deduced amino acid sequence of the full-length cDNA revealed striking homology to the product of the Chlamydomonas reinhardtii PF16 locus, which encodes a protein localized to the central pair of the flagellar axoneme. The human gene encodes 1.8- and 2.8-kb mRNAs highly expressed in testis but not in prostate, ovary, spleen, thymus, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, liver, muscle, kidney, and pancreas. The gene was mapped to chromosome 10p11.2-p12. Antibodies raised against SPAG6 sequences localized the protein to the tails of permeabilized human sperm. Both the Chlamydomonas protein and SPAG6 contain eight contiguous armadillo repeats, which place them in a family of proteins known to mediate protein-protein interactions. The cloning of the human homologue of the Chlamydomonas PF16 locus provides a new avenue to explore the role of the axoneme central pair in human sperm function. |
| Databáze: | OpenAIRE |
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