Glycoprotein 170 Induces Platelet-Activating Factor Receptor Membrane Expression and Confers Tumor Cell Hypersensitivity to NK-Dependent Cell Lysis

Autor: Christina Fleet, Yves Denizot, Jean-François Bourge, Philippe Leboulch, Rob Pawliuk, François Sigaux, Christian Berthou, Eugene Michel, Daniela Geromin, Annie Soulié, Marilyne Sasportes
Přispěvatelé: Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations (PMRIL), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Centre National de la Recherche Scientifique (CNRS), Carrion, Claire
Rok vydání: 2004
Předmět:
Cytotoxicity
Immunologic
MESH: Hydrogen-Ion Concentration
Lysis
Cell
MESH: Receptors
G-Protein-Coupled
Receptors
G-Protein-Coupled
Interleukin 21
Transduction
Genetic
Immunology and Allergy
Teratoma
MESH: Teratoma
Hydrogen-Ion Concentration
MESH: Transduction
Genetic
MESH: Drug Resistance
Neoplasm
Cell biology
Killer Cells
Natural
MESH: Retroviridae
medicine.anatomical_structure
Cell killing
Interleukin 12
[SDV.IMM]Life Sciences [q-bio]/Immunology
MESH: Leukemia
Erythroblastic
Acute
MESH: Killer Cells
Natural
ATP Binding Cassette Transporter
Subfamily B
NLM
MESH: Cell Line
Tumor
[SDV.IMM] Life Sciences [q-bio]/Immunology
Paclitaxel
Immunology
MESH: Glycoproteins
MESH: Carcinoma
Embryonal
Platelet Membrane Glycoproteins
Biology
Transfection
Carcinoma
Embryonal
Cell Line
Tumor
medicine
Humans
MESH: Paclitaxel
MESH: Cytotoxicity
Immunologic
Platelet Activating Factor
Glycoproteins
MESH: K562 Cells
MESH: Platelet Activating Factor
MESH: Humans
Lymphokine-activated killer cell
MESH: Transfection
Cell Membrane
MESH: Clone Cells
MESH: Platelet Membrane Glycoproteins
Molecular biology
Clone Cells
Retroviridae
Perforin
Drug Resistance
Neoplasm
biology.protein
Leukemia
Erythroblastic
Acute
MESH: Genes
MDR
Genes
MDR
K562 Cells
MESH: Cell Membrane
K562 cells
Zdroj: Journal of Immunology
Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2004, 172 (6), pp.3604-11
ISSN: 1550-6606
0022-1767
Popis: Multidrug resistance (MDR) confers resistance to anticancer drugs and reduces therapeutic efficiency. It is often characterized by the expression of the MDR1 gene product P-glycoprotein (or gp170) at the membrane of tumor cells. To further propose a potential complementary tool in cancer treatment, the sensitivity of gp170 tumor cells to NK-dependent lysis was investigated. Two kinds of cells were generated from wild-type K562 erythroleukemic cells: the first were derived from Taxol-selected cells and cloned, whereas the second were retrovirally transduced by the cDNA of the MDR1 gene. The last process was also applied to the human embryonal carcinoma cells called Tera-2 cells. First, both cloned and MDR-1 K562 cells appeared highly susceptible to naive NK cell killing. Interestingly, in addition, Tera-2 cells that were not sensitive to NK lysis could be killed when they expressed gp170 at their membranes. In previous data, we demonstrated that NK cell release of bimolecular complexes composed of perforin and platelet-activating factor (PAF) interacting with the PAF-R, which has to be expressed on the target cell membranes, were components of NK tumor cell killing. In the present study, we show that gp170 has the capacity to drive constitutive PAF-R expression on tumor cells, which could be responsible for hypersensitivity to NK lysis and accelerated cell death.
Databáze: OpenAIRE
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