Ferritin-mediated siRNA delivery and gene silencing in human tumor and primary cells
| Autor: | Maider Muñoz-Culla, Le Li, Mato Knez, David Otaegui, César Trigueros, Fan Yang, Lianbing Zhang, Unai Carmona, Maria Paz Lopez |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
T-Lymphocytes Biophysics Bioengineering Endogeny 02 engineering and technology Biology Peripheral blood mononuclear cell Biomaterials 03 medical and health sciences Humans Gene silencing Gene Silencing RNA Small Interfering Mesenchymal stem cell Gene Transfer Techniques Mesenchymal Stem Cells Hep G2 Cells Transfection 021001 nanoscience & nanotechnology Molecular biology Cell biology Ferritin 030104 developmental biology Mechanics of Materials Lipofectamine Ferritins Drug delivery Ceramics and Composites biology.protein Caco-2 Cells Lysosomes 0210 nano-technology |
| Zdroj: | Biomaterials. 98:143-151 |
| ISSN: | 0142-9612 |
| Popis: | We demonstrate a straightforward method to encapsulate siRNA into naturally available and unmodified human apoferritin. The encapsulation into apoferritin is independent of the sequence of the siRNA and provides superior protection for those sensitive molecules. High efficiency in transfection can be achieved in human tumorigenic cells, human primary mesenchymal stem cells (hMSC) and peripheral blood mononuclear cells (PBMCs). In contrast to Lipofectamine, highly effective gene silencing can be achieved with ferritin as the delivery agent in both tumor cells and PBMCs at low siRNA concentrations (10 nM). As an endogenous delivery agent, apoferritin does not induce immune activation of T- and B-cells in human PBMCs. Apoferritin shows intrinsic anti-inflammatory effects and apoferritin-mediated delivery shows a preference for immune-activated T- and B-cells, a natural selectivity which may turn useful for drug delivery in case of infections or inflammatory diseases. |
| Databáze: | OpenAIRE |
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