| Autor: |
David Peterhoff, Stefanie Thalhauser, Patrick Neckermann, Clara Barbey, Kristina Straub, Julian Nazet, Rainer Merkl, Gernot Laengst, Miriam Breunig, Ralf Wagner |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. |
| ISSN: |
1873-3441 |
| Popis: |
Selective targeting of germline B cells with specifically designed germline-targeting HIV-1 envelope immunogens (GT-Env) is considered a feasible vaccination strategy to elicit broadly neutralizing antibodies (bnAbs). BnAbs are extremely valuable because they neutralize genetically distant viral strains at the same time. To overcome its inherently low affinity to germline B cells, the aim of the study was to present GT-Env via different immobilization strategies densely arrayed on the surface of nanoparticles. We engineered a prefusion-stabilized GT-Env trimer with affinity to VRC01 germline B cells using a bioinformatics-supported design approach. Distinct glycan modifications and amino acid substitutions yielded a GT-Env trimer which bound to the receptor with a K |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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