Male IL-6 gene knock out mice developed more advanced osteoarthritis upon aging
| Autor: | Pieter de Hooge, Onno J. Arntz, Miranda B. Bennink, Wim B. van den Berg, Alfons S. K. de Hooge, Fons A. J. van de Loo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cartilage
Articular Male Aging medicine.medical_specialty Pathology Proteoglycan synthesis Bone density Biomedical Engineering Arthritis Osteoarthritis Auto-immunity transplantation and immunotherapy [N4i 4] Chondrocyte Mice Knock out mice Chondrocytes Sex Factors Rheumatology Bone Density Internal medicine medicine Animals Orthopedics and Sports Medicine Collagenases Tibia Mice Knockout Chronic inflammation and autoimmunity [UMCN 4.2] Bone mineral Ligaments Sclerosis Chemistry Interleukin-6 Cartilage medicine.disease Arthritis Experimental Mice Inbred C57BL Pathogenesis and modulation of inflammation [N4i 1] Endocrinology medicine.anatomical_structure Prostaglandins Female Infection and autoimmunity [NCMLS 1] Ex vivo |
| Zdroj: | Osteoarthritis and Cartilage, 13, 1, pp. 66-73 Osteoarthritis and Cartilage, 13, 66-73 |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2004.09.011 |
| Popis: | Contains fulltext : 48749.pdf (Publisher’s version ) (Closed access) OBJECTIVE: Interleukin-6 (IL-6) is expressed in osteoarthritic joints but its function in osteoarthritis (OA) is unknown. To study this, spontaneous and experimental OA were evaluated in IL-6 deficient (IL-6(-/-)) mice. DESIGN: Histology of knees of 18-23-month-old wild type (wt) and IL-6(-/-) mice was compared for signs of OA. Cartilage proteoglycan (PG) density was measured by image analysis on safranin-O stained whole knee sections. Chondrocyte PG synthesis was measured ex vivo by (35)S-sulfate incorporation. Knee bone mineral density (BMD) was measured by dual energy x-ray absorptiometry. In young mice (3 months), OA was induced by intra-articular injection of collagenase. RESULTS: The incidence of extensive cartilage loss at both lateral and medial sides was markedly higher in old IL-6(-/-) males, but not in females, as compared to their wt controls. Compared to age-matched wt mice, reduced ex vivo PG synthesis was found during aging in IL-6(-/-) males, without affecting their cartilage PG density. IL-6(-/-) males showed more extensive extracellular matrix deposition in the collateral ligaments and subchondral bone sclerosis, predominantly at the medial side. Total knee BMD decreased more in IL-6(-/-) (-23%) than in wt (-10%) males during aging. Collagenase-induced OA showed a similar degree of joint pathology in both strains, implying that OA susceptibility was not different at younger age. CONCLUSIONS: Upon aging, IL-6(-/-) male mice developed more severe spontaneous OA. Reduced PG synthesis and BMD values might be indicative for an impaired repair response in IL-6(-/-) mice. This suggests a protective role for IL-6 in age-related OA in male mice. |
| Databáze: | OpenAIRE |
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