A theoretical study of concentration of profiles of primary cytochemical-enzyme reaction products in membrane-bound cell organelles and its application to lysosomal acid phosphatase
| Autor: | P. van Duijn, W. Th. Hermens, M. Tjok Joe, W. A. L. Duijndam, C. J. Cornelisse |
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| Rok vydání: | 1976 |
| Předmět: |
Diffusion
Kinetics Acid Phosphatase Models Biological Phosphates chemistry.chemical_compound Animals chemistry.chemical_classification Chromatography biology Computers Histocytochemistry Acid phosphatase Substrate (chemistry) Cell Biology Phosphate Enzyme assay Rats Lysosomal acid phosphatase Enzyme chemistry Liver Biophysics biology.protein Anatomy Lysosomes Mathematics |
| Zdroj: | The Histochemical journal. 8(6) |
| ISSN: | 0018-2214 |
| Popis: | A numerical method was developed for computing the steady-state concentration gradient of a diffusible enzyme reaction product in a membrane-limited compartment of a simplified theoretical cell model. In cytochemical enzyme reactions proceeding according to the metal-capture principle, the local concentration of the primary reaction product is an important factor in the onset of the precipitation process and in the distribution of the final reaction product. The following variables were incorporated into the model: enzyme activity, substrate concentration, Km, diffusion coefficient of substrate and product, particle radius and cell radius. The method was applied to lysosomal acid phosphatase. Numerical values for the variables were estimated from experimental data in the literature. The results show that the calculated phosphate concentrations inside lysosomes are several orders of magnitude lower than the critical concentrations for efficient phosphate capture found in a previous experimental model study. Reasons for this apparent discrepancy are discussed. |
| Databáze: | OpenAIRE |
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