OsMTD2‐mediated reactive oxygen species (ROS) balance is essential for intact pollen‐tube elongation in rice
Autor: | Ki-Hong Jung, Soon Ki Park, Woo-Jong Hong, Yu-Jin Kim, Sun Tae Kim, Sunok Moon, Myung-Hee Kim |
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Rok vydání: | 2021 |
Předmět: |
Mutant
Stamen Germination Pollen Tube Plant Science Biology medicine.disease_cause Transcriptome Gene Expression Regulation Plant Two-Hybrid System Techniques Pollen Genetics medicine Gene Plant Proteins chemistry.chemical_classification Reactive oxygen species Cell Membrane food and beverages Oryza Cell Biology Plants Genetically Modified Sperm Cell biology chemistry Mutation Pollen tube Reactive Oxygen Species Protein Processing Post-Translational |
Zdroj: | The Plant Journal. 107:1131-1147 |
ISSN: | 1365-313X 0960-7412 |
Popis: | The highly specialized haploid male gametophyte-pollen consist of two sperm cells and a large vegetative cell. Successful fertilization requires proper growth timing and rupture of the pollen tube until it delivers sperm cells, which occur immediately after a pollen grain hydrates. Although a tight regulation on polar cell-wall expansion of the pollen tube is fundamentally important, the underlying molecular mechanism remains largely unknown, especially in crop plants. Here, we characterized the function of male-gene transfer defective 2 (OsMTD2) gene in rice (Oryza sativa), which belongs to the plant-specific receptor-like kinase, the CrRLK1L family. We demonstrated that OsMTD2 is an essential male factor participating in pollen-tube elongation based on genetic evidence and physiological observations. Because of unavailability of homozygous mutant via conventional methods, we used CRISPR-Cas9 system to obtain homozygous knockout mutant of OsMTD2. We were able to identify phenotypic changes including male sterility due to early pollen-tube rupture in the mutant. We observed that the production of reactive oxygen species (ROS) was dramatically reduced in mutants of OsMTD2 pollen grain and tubes with defective pectin distribution. Transcriptome analysis of osmtd2-2 versus wild-type anthers revealed that genes involved in defense responses, metabolic alteration, transcriptional and protein modification were highly upregulated in the osmtd2-2 mutant. Through yeast-two-hybrid screening, we found that OsMTD2 kinase interacts with E3 ligase SPL11. Taken together, we propose that OsMTD2 has crucial functions in promoting pollen-tube elongation through cell-wall modification, possibly by modulating ROS homeostasis during pollen-tube growth. |
Databáze: | OpenAIRE |
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