Morphologic and transcriptomic assessment of bovine embryos exposed to dietary long-chain fatty acids
| Autor: | Divakar J. Ambrose, A. Behrouzi, Marcos G. Colazo, Stephen Tsoi, Michael K. Dyck, Reza Salehi, Masahito Oba, Benjamin K. Tsang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Embryology animal structures Linolenic acid Linoleic acid Biology Cattle feeding Andrology Transcriptome 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Animals Dairy cattle chemistry.chemical_classification 030219 obstetrics & reproductive medicine Gene Expression Profiling Fatty Acids Embryogenesis food and beverages Obstetrics and Gynecology Embryo Cell Biology Embryo Mammalian 030104 developmental biology Gene Expression Regulation Reproductive Medicine chemistry Dietary Supplements Cattle Female Polyunsaturated fatty acid |
| Zdroj: | Reproduction. 152:715-726 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-16-0093 |
| Popis: | The main objectives of this study were to determine the influence of diets enriched in α-linolenic, linoleic or oleic acid on the development and transcriptomic profile of embryos collected from dairy cattle. Non-lactating Holstein cows received one of the three diets supplemented with 8% rolled oilseeds: flax (FLX,n= 8), sunflower (SUN,n= 7) or canola (CAN,n= 8). After a minimum 35-day diet adaptation, cows were superovulated, artificially inseminated and ova/embryos recovered non-surgically after 7.5 days. Cows fed FLX had less degenerated embryos and more viable embryos than those fed CAN or SUN. In total, 175 genes were differentially expressed in blastocysts from cows fed FLX than in cows fed CAN or SUN. These differentially expressed genes were mainly involved in cellular growth and proliferation, cellular development, and cell survival and viability. In conclusion, dietary n-3 polyunsaturated fatty acids reduced early embryonic degeneration possibly through improving embryonic cell survival and viability. |
| Databáze: | OpenAIRE |
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