The Potyviral P3 Protein Targets Eukaryotic Elongation Factor 1A to Promote the Unfolded Protein Response and Viral Pathogenesis
| Autor: | Xin Chen, Pradeep Kachroo, Hexiang Luan, Haijan Zhi, Na Ma, Xiaoyan Cui, Aardra Kachroo, M.B. Shine |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Viral pathogenesis Potyvirus Plant Science Biology Virus Replication 03 medical and health sciences Viral Proteins Peptide Elongation Factor 1 Gene Expression Regulation Plant Mosaic Viruses Sequence Homology Nucleic Acid Genetics Gene silencing Protein Isoforms Amino Acid Sequence Host factor Plant Diseases Plant Proteins Base Sequence Sequence Homology Amino Acid Virulence Reverse Transcriptase Polymerase Chain Reaction Endoplasmic reticulum fungi food and beverages Articles Endoplasmic Reticulum Stress Elongation factor 030104 developmental biology Viral replication Gene Knockdown Techniques Host-Pathogen Interactions Unfolded protein response Unfolded Protein Response Soybeans Nuclear localization sequence |
| Zdroj: | Plant physiology. 172(1) |
| ISSN: | 1532-2548 |
| Popis: | The biochemical function of the potyviral P3 protein is not known, although it is known to regulate virus replication, movement, and pathogenesis. We show that P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean. Eukaryotic elongation factor 1A (eEF1A), a well-known host factor in viral pathogenesis, is essential for SMV virulence and the associated unfolded protein response (UPR). Silencing GmEF1A inhibits accumulation of SMV and another ER-associated virus in soybean. Conversely, endoplasmic reticulum (ER) stress-inducing chemicals promote SMV accumulation in wild-type, but not GmEF1A-knockdown, plants. Knockdown of genes encoding the eEF1B isoform, which is important for eEF1A function in translation elongation, has similar effects on UPR and SMV resistance, suggesting a link to translation elongation. P3 and GmEF1A promote each other’s nuclear localization, similar to the nuclear-cytoplasmic transport of eEF1A by the Human immunodeficiency virus 1 Nef protein. Our results suggest that P3 targets host elongation factors resulting in UPR, which in turn facilitates SMV replication and place eEF1A upstream of BiP in the ER stress response during pathogen infection. |
| Databáze: | OpenAIRE |
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