Effect of hyperbaric oxygenation on the expression of glutathione peroxidase 4 and lactoperoxidase genes in the lung of isogenic mice after ischemia/reperfusion injury in the small bowel
| Autor: | Alexandre Key WakateTeruya, Djalma José Fagundes, José Carlos Chaves, Adauto Tsutomu Ikejiri, Frederico Somaio Neto, Cândida Aparecida Leite Kassuya, Paulo Roberto Bertoletto, Murched Omar Taha |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant RD1-811 medicine.medical_treatment Ischemia Gene Expression Pharmacology GPX4 medicine.disease_cause Antioxidants 03 medical and health sciences Mice Laparotomy medicine Animals Lactoperoxidase Lung Glutathione Peroxidase Hyperbaric Oxygenation business.industry medicine.disease Phospholipid Hydroperoxide Glutathione Peroxidase Intestines Disease Models Animal Oxidative Stress 030104 developmental biology medicine.anatomical_structure Reperfusion Injury Surgery business Reperfusion injury Oxidative stress |
| Zdroj: | Acta Cirurgica Brasileira, Vol 33, Iss 5, Pp 462-471 (2018) Acta Cirúrgica Brasileira v.33 n.5 2018 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
| ISSN: | 1678-2674 |
| Popis: | Purpose: To evaluate the effect of hyperbaric oxygenation (HBO) on the expression of the genes antioxidant glutathione peroxidase 4 (Gpx4) and lactoperoxidase (Lpo) in the lung of mice subjected to intestinal ischemia and reperfusion (IIR). Methods: Control group (CG) in which were subjected to anesthesia, laparotomy and observation for 120 minutes; an ischemia and reperfusion group (IRG) subjected to anesthesia, laparotomy, small bowel ischemia for 60 minutes and reperfusion for 60 minutes; and three groups treated with HBO during ischemia (HBOG + I), during reperfusion (HBOG + R) and during ischemia and reperfusion (HBOG + IR). Studied 84 genes of oxidative stress by the method (RT-qPCR). Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student’s t-test p |
| Databáze: | OpenAIRE |
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