Morphokinetics of human blastocyst expansion in vitro
| Autor: | Hyeong Jun Ahn, Thomas T. F. Huang, K. Chinn, Bruce Kessel, Thomas S. Kosasa |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine Embryonic Development Biology Time-Lapse Imaging Embryo Culture Techniques Andrology Young Adult 03 medical and health sciences 0302 clinical medicine Embryo Culture Technique medicine Humans Embryo Implantation Sperm Injections Intracytoplasmic Blastocyst Retrospective Studies 030219 obstetrics & reproductive medicine Embryogenesis Blastocoel Obstetrics and Gynecology Embryo Anatomy Embryo Transfer In vitro Embryo transfer Kinetics 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Embryoscope Oocytes Regression Analysis Female Algorithms Developmental Biology |
| Zdroj: | Reproductive BioMedicine Online. 33:659-667 |
| ISSN: | 1472-6483 |
| DOI: | 10.1016/j.rbmo.2016.08.020 |
| Popis: | Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities. Two types of oscillations were distinguished. An E-Type ('expansion') had positive peak and positive or slightly negative trough interval rate of change values, and these characterized most of the expansion period. A C-type ('contraction') represented an infrequent but notable contraction of the blastocoel with loss of blastocoel fluid. These were reversible within 2-4 h in both groups and followed by further expansion. Therefore, oscillatory pulses are an intrinsic property of the trophectoderm. The zona seems to variably dampen the amplitude of these pulses. Expansion kinetics were compared between blastocysts with known positive (KID+) or negative (KID-) implantation outcomes. Regression analysis suggests that expansion may be relatively restricted in KID- embryos blastulating at relatively later times. These data extend observations in other mammalian systems and may provide information useful for clinical selection algorithms. |
| Databáze: | OpenAIRE |
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