Electrochemiluminescence Energy Resonance Transfer System between RuSi Nanoparticles and Hollow Au Nanocages for Nucleic Acid Detection
| Autor: | Hong-Yuan Chen, Yin-Zhu Wang, Jing-Juan Xu, Jian-Bin Pan, Hai-Jie Lu, Si-Yuan Ji, Xiliang Luo, Wei Zhao |
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| Rok vydání: | 2018 |
| Předmět: |
Luminescence
Absorption spectroscopy Nanoparticle Biosensing Techniques 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Analytical Chemistry Nanocages Nucleic Acids Electrochemiluminescence Surface plasmon resonance Plasmon Chemistry DNA Electrochemical Techniques Surface Plasmon Resonance 021001 nanoscience & nanotechnology Acceptor Nanostructures 0104 chemical sciences MicroRNAs Energy Transfer Gold 0210 nano-technology Biosensor |
| Zdroj: | Analytical Chemistry. 90:10434-10441 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.8b02347 |
| Popis: | This paper describes an electrochemiluminescence resonance energy transfer (ECL-RET) system using Ru(bpy)32+-doped silica nanoparticles (RuSi NPs) as the ECL donor and hollow Au nanocages as the ECL acceptor. Tetrahedron DNA (TD) was used to construct the biosensing interface and control the distance (4.8 nm) between the ECL donor–acceptor pairs. The surface plasmon resonance (SPR) nanostructures, Au nanocages were assembled via the hairpin based sandwich assay. Due to the well overlap between the plasmon absorption spectrum of Au nanocages (628 nm) and the ECL emission spectrum of RuSi NPs (620 nm), high efficient energy transfer could occur. Subsequent cyclic DNA amplification further increased the binding amount of Au nanocages. Since the ECL inhibition is closely related with the binding amount of Au nanocages, a general “signal-off” ECL bioassay could thus be tailored with high sensitivity. At the optimized conditions, this ECL-RET system performed well with great stability and repeatability for nucl... |
| Databáze: | OpenAIRE |
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