Muscle specific versus ubiquitous expression of Gag based HIV-1 DNA vaccines: a comparative analysis
| Autor: | Diana Hammer, Ralf Wagner, Alexandra Bojak, Hans Wolf |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cellular immunity
Lung Neoplasms Transcription Genetic HIV Core Protein p24 Cytomegalovirus Gene Products gag HIV Antibodies Biology DNA vaccination Mice Immune system Antigen Antibody Specificity Genes Reporter Gene expression Genes Synthetic Tumor Cells Cultured Vaccines DNA Animals Humans Vector (molecular biology) Protein Precursors Luciferases Muscle Skeletal Promoter Regions Genetic Creatine Kinase AIDS Vaccines Mice Inbred BALB C Vaccines Synthetic General Veterinary General Immunology and Microbiology Immunogenicity Public Health Environmental and Occupational Health Creatine Kinase MM Form Promoter Genes gag Virology Isoenzymes Enhancer Elements Genetic Infectious Diseases Organ Specificity HIV-1 Molecular Medicine Female |
| Zdroj: | Vaccine. 20:1975-1979 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(02)00081-6 |
| Popis: | Most studies on DNA-based immunization have used viral promoters to drive antigen expression. In this study, we analyzed the properties of the commonly used CMV promoter, the tissue specific murine muscle creatine kinase (MCK) promoter as well as a hybrid MCK/CMV promoter regarding promoter activity and tissue specificity in vitro. Furthermore, the efficiency of inducing HIV-1 Gag specific immune responses in vivo following intramuscular immunization of nacked DNA containing a codon optimized synthetic gene was compared. Although antibody titers and cellular immune responses using the MCK construct were slightly reduced as compared to convential CMV based vector modules, the utilization of nonviral promoters may add significantly to the safety of future DNA vaccines. |
| Databáze: | OpenAIRE |
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