Induction of Apoptosis by Three Marine Algae through Generation of Reactive Oxygen Species in Human Leukemic Cell Lines
| Autor: | Huey-Lan Huang, Shwu-Li Wu, Ray-Ling Huang, Hui-Fen Liao, Yuh-Cheng Yang, Yu-Jen Chen, Chii-Ming Jiang, Yu-Yawn Chen |
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| Rok vydání: | 2005 |
| Předmět: |
Carcinoma
Hepatocellular Apoptosis HL-60 Cells Biology Phaeophyta Flow cytometry chemistry.chemical_compound Chlorophyta Tumor Cells Cultured medicine Humans Colpomenia sinuosa chemistry.chemical_classification Reactive oxygen species U937 cell medicine.diagnostic_test Superoxide Liver Neoplasms Eukaryota General Chemistry Molecular biology chemistry Biochemistry Leukemia Myeloid Cell culture Rhodophyta Apoptotic signaling pathway Reactive Oxygen Species General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 53:1776-1781 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf049445n |
| Popis: | In this study, we examined the antitumor effect of marine algae extracts on human hepatoma and leukemia cells. Ethyl acetate extracts from Colpomenia sinuosa (Cs-EA), Halimeda discoidae (Hd-EA), and Galaxaura oblongata (Go-EA) directly inhibited the growth of human hepatoma HuH-7 cells and leukemia U937 and HL-60 cells in a time- and dose-dependent manner. Specifically, these algae extracts induced apoptosis of U937 and HL-60 cells as evaluated by detection of hypodiploid cells using flow cytometry and observation of condensed and fragmented nuclei in algae extract-treated cells. Intracellular reactive oxygen species (ROS), especially hydrogen peroxide and superoxide anion, were increased about 2-3-fold in U937 cells treated with Cs-EA for 3-5 h. Interestingly, antioxidant N-acetylcysteine effectively blocked Cs-EA-, Hd-EA-, and Go-EA-induced apoptosis, suggesting that ROS is a key mediator in the apoptotic signaling pathway. In conclusion, our results show that algae extracts induce apoptosis in human leukemia cells through generation of ROS. |
| Databáze: | OpenAIRE |
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