In vitro sensitivity of forest soil enzymes to temperature increase in Western Patagonia
| Autor: | Neal B. Stolpe, Ana María Bonilla, Daniel Chávez, Katerin Almendras, Ángela Machuca, Juan A. Barrera, Carolin Córdova |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Soil Science Plant Science global warming 01 natural sciences Activation energy N-acetyl-β-glucosaminidase chemistry.chemical_classification biology β-glucosidase Soil classification 04 agricultural and veterinary sciences Mineralization (soil science) Enzyme assay In vitro Enzyme Agronomy chemistry N-Acetyl-beta-glucosaminidase 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries C mineralization Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Journal of soil science and plant nutrition, Volume: 18, Issue: 1, Pages: 202-219, Published: MAR 2018 Journal of soil science and plant nutrition v.18 n.1 2018 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | Soil enzymes can be sensitive indicators of C changes in forest soils produced by global warming. This study assessed the response to temperature of soil enzymes in forest soils from the fragile cold ecosystems of Western Patagonia (Chile), to be used as indicators of the biological C emissions from the soil under climate change for this Region. Soil from two sites of Nothofagus forest were sampled (0-5 cm), and soil C mineralization (21 days of soil incubation) and the enzyme activities of N-acetyl-β-glucosaminidase (NGA, linked to C and N cycles), β-glucosidase (BG, linked to C cycle), and endo-1,4-β-glucanase (CMCase, linked to C cycle) were measured at 5°C and 20oC. Additionally, sensitivity of enzyme activities was measured at 0, 5, 10 and 20oC during experimental protocol incubation procedure, and energy activation (Ea) and Q10 parameters were calculated. At both sites, the NGA activity mirrored the microbial activity, when temperature rose from 5 ºC to 20 °C. The NGA showed Ea higher than BG, indicating that the release of N-acetyl-glucosamine was more sensitive to temperature than the release of glucose, related to BG. The results suggest the applicability of NGA as potential indicator to assess effects of global warming in forest soils from Patagonia. |
| Databáze: | OpenAIRE |
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