Upregulated expression of postsynaptic density-93 and N-methyl-d-aspartate receptors subunits 2B mRNA in temporal lobe tissue of epilepsy
| Autor: | Yuping Wang, Feng-Ying Liu, Yun-lin Li, Xuefeng Wang, Ming-Wei Li, Ji-Jun Sun, Jin-mei Li, Jianguo Zhang, Guoming Luan, Zhiqin Xi |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Adolescent Biophysics Biology Bioinformatics Receptors N-Methyl-D-Aspartate Biochemistry Pathogenesis Epilepsy Complementary DNA medicine Humans Child Molecular Biology Messenger RNA Microarray analysis techniques Tumor Suppressor Proteins musculoskeletal neural and ocular physiology Intracellular Signaling Peptides and Proteins Brain Membrane Proteins Cell Biology Middle Aged medicine.disease Molecular biology Temporal Lobe Up-Regulation Reverse transcription polymerase chain reaction nervous system Child Preschool Female GAPDH Gene Signal transduction Disks Large Homolog 4 Protein Guanylate Kinases psychological phenomena and processes |
| Zdroj: | Biochemical and Biophysical Research Communications. 358:825-830 |
| ISSN: | 0006-291X |
| Popis: | To investigate the expression of PSD-93 mRNA and NR2B mRNA in the brain tissue from the patients with epilepsy so as to explore the possible mechanisms of the pathogenesis of the epilepsy.Fifty-six patients with epilepsy were divided into intractable epilepsy (IE) and non-intractable epilepsy (NIE) groups. cDNA microarrays prepared from the brain tissues obtained from these two groups were scanned and comparison to those from the non-epileptogenic control (C) was made. Expression level of PSD-93mRNA and NR2BmRNA were examined by reverse transcription polymerase chain reaction (GAPDH gene, internal control). Expression ratio (target gene/GAPDH) was used to evaluate each gene relative expression level.The cDNA microarray analysis showed that the expression of PSD-93 mRNA related to the function of NMDAR-NO signal transduction pathway was significantly higher in epilepsy patients than those in the controlled group. The results of RT-PCR were consistent with those of the cDNA microarrays. The relative expression ratio of PSD-93 in patients with non-epileptogenic control, NIE, and IE was 0.159, 0.368, and 0.341, respectively. Correspondingly, that of NR2B was 0.198, 0.738, and 0.903, respectively. The expressions of PSD-93 and NR2B in the NIE and IE were significantly higher than those of control, respectively (P0.05). However, there was no significantly difference the expression of PSD-93 between NIE and IE. (P0.05), neither do that of NR2B (P0.05).The upregulated expressions of PSD-93 mRNA and NR2BmRNA may be involved in the pathogenesis of epilepsy. |
| Databáze: | OpenAIRE |
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