Use of Molecular Beacons To Detect an Antifolate Resistance-Associated Mutation in Plasmodium falciparum

Autor:	Jobin Eslahpazire, Jean-Francois Delabre, Jacques Le Bras, Rémy Durand, Anne Marmorat-Khuong, Jean-Philippe di Piazza, Sayeh Jafari
Jazyk:	angličtina
Rok vydání:	2000
Předmět:	Plasmodium falciparum Drug Resistance Drug resistance Polymerase Chain Reaction law.invention Apicomplexa law Molecular beacon Mechanisms of Resistance parasitic diseases Animals Humans Pharmacology (medical) Polymerase chain reaction Pharmacology Genetics biology Point mutation Nucleic acid sequence biology.organism_classification Tetrahydrofolate Dehydrogenase Infectious Diseases Mutation Folic Acid Antagonists Restriction fragment length polymorphism Polymorphism Restriction Fragment Length
Popis:	A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum . One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26fe524ba3b81672df1ce43dcc72ddb3 https://europepmc.org/articles/PMC90225/ Zobrazit plný text záznamu