Parkin Null Cortical Neuronal/Glial Cultures are Resistant to Amyloid-β1-42 Toxicity: A Role for Autophagy?
| Autor: | Justo García de Yébenes, Ana M. Gómez, Juan Perucho, Maria Angeles Mena, Rosa M. Solano, María José Casarejos |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Proteasome Endopeptidase Complex
Programmed cell death Cell Survival Ubiquitin-Protein Ligases Blotting Western tau Proteins Parkin Mice Autophagy medicine Animals Protein kinase B Cells Cultured Cerebral Cortex Mice Knockout Neurons Glutathione Peroxidase Amyloid beta-Peptides Chemistry General Neuroscience Ubiquitination Wild type General Medicine medicine.disease Glutathione Immunohistochemistry Peptide Fragments Culture Media Astrogliosis Cell biology Psychiatry and Mental health Clinical Psychology Proteasome Geriatrics and Gerontology Signal transduction Neuroglia Molecular Chaperones |
| Zdroj: | Journal of Alzheimer's Disease. 32:57-76 |
| ISSN: | 1875-8908 1387-2877 |
| DOI: | 10.3233/jad-2012-120406 |
| Popis: | Dementia occurs often in late stages of Parkinson's disease (PD) but its cause is unknown. Likewise there is little information about the interaction between proteins that produce PD and those implicated in Alzheimer's disease (AD). Here we have investigated the interactions between parkin protein and the amyloid-β (Aβ)1-42 peptide. We examined the effects of oligomeric Aβ1-42 peptide on the survival, differentiation, and signaling pathways in cortical cultures from wild type (WT) and parkin null (PK-KO) mice. We discovered that PK-KO cells were more resistant than WT to Aβ1-42. This peptide induced neuronal cell death, astrogliosis, microglial proliferation, and increased total and hyperphosphorylated tau and levels of chaperones HSP-70 and CHIP in WT, but not in Aβ-treated PK-KO cultures. Aβ1-42 decreased proteasome activities in WT and PK-KO cultures, but the ubiquitination of proteins only increased in WT cultures. Aβ1-42 induced a short activation of ERK1/2 and AKT signaling pathways, implicated in cell survival, in PK-KO-treated cells, and a shift in the autophagy marker LC3-II/LC3-I ratio. In addition, the percentage of cells immunoreactive to both HSC70 and LAMP-2A increased in PK-KO cultures versus WT and furthermore in PK-KO cultures treated with Aβ1-42. Pre-treatment with inhibitors of glutathione synthesis or autophagy reverted the resistance to Aβ1-42 of the PK-KO cultures. In conclusion, the loss of parkin protein triggers the compensatory mechanisms of cell protection against Aβ1-42. Parkin suppression, therefore, is not a risk factor for dementia of AD type. |
| Databáze: | OpenAIRE |
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