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Introduction:From ancient period onwards Triphala and its combinations were used in liver disorders in Ayurveda, but its mechanism of action are not still evaluated. Triphala is the combination in equi-proportion 1:1:1, of dried fruits of Amalaki (Phyllanthus emblica Linn), Bibhitaki (Terminalia bellerica Roxb) and Haritaki (Terminalia chebula Retz). The present study evaluated the hepatoprotective effect of methanolic extract of Triphala (T) and its different combinations such as Triphala with honey [TM], Triphala with Pippali (dried fruit of Piper longum Linn) [TP] and Triphala with Yashtimadhu (rhizhomes of Glycyrrhiza glabra Linn) [TY] in HepG2 cell lines using MTT assay against acetaminophen induced hepato toxicity. Flow cytometry was used to count the viable cells. Methodology:HepG2 ( Human hepatic carcinoma) cell line was purchased from NCCS Pune and maintained in Dulbecco?s modified eagle?s media. Then T, TM, TP and TY were added in to acetaminophen induced hepatotoxicity cells at five concentrations; 6.25?g/ml, 12.5 ?g/ml, 25 ?g/ml, 50 ?g/ml and 100 ?g/ml using MTT assay [(3-(4,5 dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay]; untreated cells and acetaminophen treated cells kept as controls. Results:The acetaminophen treated cells showed 34.15% viability in MTT assay. The T, TM, TP and TY treated groups showed increase in the percentage cell viability at 6.25?g/ml, 12.5 ?g/ml, 25 ?g/ml, 50 ?g/ml and 100 ?g/ml concentrations and the result were significant (p |
