Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source
| Autor: | James M. Holton, Scott Classen, Greg L. Hura, Michal Hammel, Ivan Rodic, John A. Tainer, George Meigs, Kevin Dyer, Patrick McGuire, Robert P. Rambo, Kenneth A. Frankel |
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| Rok vydání: | 2013 |
| Předmět: |
Diffraction
Materials science small-angle X-ray scattering (SAXS) Nanotechnology 7. Clean energy General Biochemistry Genetics and Molecular Biology macromolecular crystallography (MX) law.invention 03 medical and health sciences 0302 clinical medicine Optics Light source law 030304 developmental biology Monochromator SIBYLS 0303 health sciences Scattering Small-angle X-ray scattering business.industry Macromolecular crystallography Research Papers Synchrotron 3. Good health Beamline business synchrotron beamlines 030217 neurology & neurosurgery |
| Zdroj: | Journal of Applied Crystallography |
| ISSN: | 0021-8898 |
| DOI: | 10.1107/s0021889812048698 |
| Popis: | The SIBYLS beamline of the Advanced Light Source at Lawrence Berkeley National Laboratory is a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline. Key features and capabilities are described along with implementation and performance. The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world’s mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B4C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources. |
| Databáze: | OpenAIRE |
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