The Genetic Program of Pancreatic β-Cell Replication In Vivo
| Autor: | Benjamin Glaser, Oriel Friedlich, Agnes Klochendler, Shalev Itzkovitz, Aharon Helman, Yuval Nevo, Maya Moran, Yuval Dor, Noa Corem, Sharona Elgavish, Inbal Caspi, Amir Eden |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Mice Transgenic 030209 endocrinology & metabolism In situ hybridization Biology Transcriptome Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Insulin-Secreting Cells Gene expression Internal Medicine Animals Gene Cell Proliferation Membrane Potential Mitochondrial Regulation of gene expression RNA Genetics/Genomes/Proteomics/Metabolomics Cell cycle Flow Cytometry Molecular biology 030104 developmental biology Gene Expression Regulation Cell Division |
| Zdroj: | Diabetes |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | The molecular program underlying infrequent replication of pancreatic β-cells remains largely inaccessible. Using transgenic mice expressing green fluorescent protein in cycling cells, we sorted live, replicating β-cells and determined their transcriptome. Replicating β-cells upregulate hundreds of proliferation-related genes, along with many novel putative cell cycle components. Strikingly, genes involved in β-cell functions, namely, glucose sensing and insulin secretion, were repressed. Further studies using single-molecule RNA in situ hybridization revealed that in fact, replicating β-cells double the amount of RNA for most genes, but this upregulation excludes genes involved in β-cell function. These data suggest that the quiescence-proliferation transition involves global amplification of gene expression, except for a subset of tissue-specific genes, which are “left behind” and whose relative mRNA amount decreases. Our work provides a unique resource for the study of replicating β-cells in vivo. |
| Databáze: | OpenAIRE |
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