Reporter Virus Neutralization Test Evaluation for Dengue and Zika Virus Diagnosis in Flavivirus Endemic Area
| Autor: | Eliana Vieira Pinto da Silva, Liliane Leal das Chagas, Raimunda do Socorro da Silva Azevedo, Rommel Mario Rodrigues Burbano, Pei Yong Shi, Bruno Tardelli Diniz Nunes, Jannifer Oliveira Chiang, Daniele Barbosa de Almeida Medeiros, Tais R Silva, Jannyce G. C Nunes, Lívia Carício Martins, Maria Helena Rodrigues de Mendonça, Livia Medeiros Neves Casseb, Daniele Freitas Henriques, Pedro Fernando da Costa Vasconcelos, Adriana Freitas Moraes, Chao Shan, Franco A. e Silva |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) diagnosis viruses Secondary infection 030231 tropical medicine Virus Neutralization Article reporter virus Serology Dengue fever Zika virus Dengue 03 medical and health sciences V?rus Rep?rter 0302 clinical medicine flavivirus Immunology and Allergy Medicine Seroconversion Molecular Biology neutralization test ZIKV General Immunology and Microbiology biology business.industry V?rus da Dengue / patogenicidade Outbreak biology.organism_classification medicine.disease Virology Zika virus / patogenicidade Flavivirus 030104 developmental biology Infectious Diseases Testes de Neutraliza??o / m?todos Flavivirus / isolamento & purifica??o business |
| Zdroj: | Pathogens; Volume 10; Issue 7; Pages: 840 Pathogens, Vol 10, Iss 840, p 840 (2021) Pathogens Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 2076-0817 2016-0135 |
| DOI: | 10.3390/pathogens10070840 |
| Popis: | Ministry of Health of Brazil and by the grants from CNPq (process 303999/2016-0 and 440405/2016-5) and CAPES (Zika fast track project). Pan American Health Organization SCON2016-01353, NIH grants AI142759, AI127744, and AI136126, and awards from the Kleberg Foundation, John S. Dunn Foundation, Amon G. Carter Foundation, Gilson Longenbaugh Foundation, and Summerfield Robert Foundation. University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / State University of Par?. Post Graduation Program in Parasitary Biology in the Amazon. Bel?m, PA, Brazil. University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Science and Health Institute. Bel?m, PA, Brazil. Federal University of Par?. Biological Sciences Institute. Bel?m, PA, Brazil. University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA . University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / State University of Par?. Post Graduation Program in Parasitary Biology in the Amazon. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology. |
| Databáze: | OpenAIRE |
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