Effects of arginine vasopressin and extracellular osmolarity on atrial natriuretic peptide release by superfused rat atria
| Autor: | J. C. Legrand, Marie A. Zongaso, Geneviève Maistre, Christiane Barthélemy, F. Masson, Alain Carayon, Michel Komajda, Eric Noé, Joëlle Eurin |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Vasopressin medicine.medical_specialty Arginine medicine.drug_class Heart Ventricles Neuropeptide Atrial Function Right In Vitro Techniques Peptide hormone Biology Membrane Potentials Potassium Chloride chemistry.chemical_compound Atrial natriuretic peptide Internal medicine medicine Animals Ventricular Function Heart Atria Pharmacology Osmotic concentration Osmolar Concentration Heart Rats Inbred Strains Atrial Function Receptor antagonist Rats Arginine Vasopressin EGTA Endocrinology chemistry cardiovascular system Calcium Calcium Channels Extracellular Space Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists |
| Zdroj: | European Journal of Pharmacology. 209:45-55 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/0014-2999(91)90009-f |
| Popis: | This study investigated the characteristics of atrial natriunetic peptide (ANP) release from superfused sliced atria and ventricles of rats. Right atria spontaneously released more immunoreactive ANP (Ir-ANP: pg/min per mg tissue) (32 ± 3) than did left atria (11 ± 2) or right ventricles (1.5 ± 0.5). Addition of 10 −9 to 10 −5 M of arginine vasopressin (AVP) to the superfusing fluid or increasing its osmolarity (290 to 490 mOsM) resulted in a significant increase of the Ir-ANP outflow from right atria. The effect of AVP was prevented by a specific V 1 receptor antagonist, ([d(dh 2 ) 5 Tyr(Me)]AVP). Superfusion with indomethacin (10 −5 M) did not alter spontaneous release but inhibited the peak levels of Ir-ANP induced by AVP (10 −5 M). Moreover, DDAVP, a specific V 2 receptor agonist, did not induce Ir-ANP release. Ca 2+ -free medium alone or plus 1 mM EGTA induced a significant increase in basal Ir-ANP outflow. The Ir-ANP released chromatographed similarly to the standard α-rANP. These results suggest a specific stimulatory effect of AVP and osmolarity and a negative influence of extracellular Ca 2+ on atrial spontaneous Ir-ANP release. It appears that the effect of AVP could be mediated by prostaglandin synthesis. |
| Databáze: | OpenAIRE |
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