Real-time polymerase chain reaction for the qualitative and quantitative detection of Mycoplasma gallisepticum
| Autor: | D R Mekkes, A Feberwee |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Mycoplasma gallisepticum
Detection limit General Immunology and Microbiology Mycoplasma meleagridis Single sample Mycoplasma synoviae Biology biology.organism_classification Virology Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Real-time polymerase chain reaction Food Animals Species Specificity law Mycoplasma imitans Animals Animal Science and Zoology Mycoplasma Infections Chickens Polymerase chain reaction Poultry Diseases |
| Zdroj: | Avian pathology : journal of the W.V.P.A. 34(4) |
| ISSN: | 0307-9457 |
| Popis: | The aim of this study was to evaluate the sensitivity and the detection limit of a real-time polymerase chain reaction (Q-PCR) developed for the qualitative and quantitative detection of Mycoplasma gallisepticum. No cross-reactivity was observed with DNA from other important avian mycoplasmas, including Mycoplasma synoviae and Mycoplasma meleagridis. However, the Q-PCR could not distinguish between M. gallisepticum and Mycoplasma imitans. The Q-PCR had detection limits 10 to 1000 times lower than a conventional commercial PCR method and than culture. The Q-PCR was used quantitatively by incorporating a set of external M. gallisepticum DNA standards, derived from a M. gallisepticum log-phase culture of a known concentration. The number of colony-forming unit equivalents per millilitre in tracheal swabs from experimentally infected birds could be determined from a single sample. The method had good reproducibility and correlated well with standard counting techniques using culture. It can be concluded that the Q-PCR described is suitable for qualitative and quantitative detection of M. gallisepticum in clinical samples. |
| Databáze: | OpenAIRE |
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