Determination of Rifampin Concentrations by Urine Colorimetry and Mobile Phone Readout for Personalized Dosing in Tuberculosis Treatment
| Autor: | Museveni Justine, Mohammad H. Al-Shaer, Tania A Thomas, Charles A. Peloquin, Sarah Criddle, Christopher Vinnard, Stellah G. Mpagama, Estomih Mduma, Scott K. Heysell, Daniel Van Aartsen, Isaac Zentner, Prakruti Rao, Claire Szipszky |
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| Rok vydání: | 2020 |
| Předmět: |
Urinalysis
Antitubercular Agents Cmax Urine Colorimetry (chemical method) 03 medical and health sciences 0302 clinical medicine Pharmacokinetics Humans Tuberculosis Medicine 030212 general & internal medicine Dosing Child 0303 health sciences Chromatography medicine.diagnostic_test Receiver operating characteristic 030306 microbiology business.industry Original Articles General Medicine Infectious Diseases Therapeutic drug monitoring Pediatrics Perinatology and Child Health Colorimetry Rifampin business Cell Phone |
| Zdroj: | J Pediatric Infect Dis Soc |
| ISSN: | 2048-7207 |
| DOI: | 10.1093/jpids/piaa024 |
| Popis: | Background Individual pharmacokinetic variability is a driver of poor tuberculosis (TB) treatment outcomes. We developed a method for measurement of rifampin concentrations by urine colorimetry and a mobile phone photographic application to predict clinically important serum rifampin pharmacokinetic measurements in children treated for TB. Methods Among spiked urine samples, colorimetric assay performance was tested with conventional spectrophotometric and the mobile phone/light box methods under various environmental and biologic conditions. Urine rifampin absorbance (Abs) was then determined from timed specimens from children treated for TB in Tanzania, and compared to serum pharmacokinetic measurements collected throughout the dosing interval. Results Both the mobile phone/light box and spectrophotometry demonstrated excellent correlation across a wide range of urine rifampin concentrations (7.8–1000 mg/L) in intra- and interday trials, 24-hour exposure to ambient light or darkness, and varying urinalysis profiles (all r ≥ 0.98). In 12 Tanzanian children, the urine mobile phone/light box measurement and serum peak concentration (Cmax) were significantly correlated (P = .004). Using a Cmax target of 8 mg/L, the area under the receiver operating characteristic curve was 80.1% (range, 47.2%–100%). A urine mobile phone/light box threshold of 50 Abs correctly classified all patients (n = 6) with serum measurements below target. Conclusions The urine colorimetry with mobile phone/light box assay accurately measured rifampin absorbance in varying environmental and biological conditions that may be observed clinically. Among children treated for TB, the assay was sensitive for detection of low rifampin serum concentrations. Future work will identify the optimal timing for urine collection, and operationalize use in TB-endemic settings. |
| Databáze: | OpenAIRE |
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