Perylenequinone-based 'turn on' fluorescent probe for hydrogen sulfide with high sensitivity in living cells
| Autor: | Qi Zhang, Yi-Meng Duan, Ju-Peng Chen, Shaojin Chen, Zhi-Qiang Hu, Wen-Jun Zheng, Kun-Peng Wang, Qianshou Zong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Hydrogen sulfide 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Fluorescence Analytical Chemistry HeLa Turn (biochemistry) chemistry.chemical_compound Limit of Detection Humans Hydrogen Sulfide Perylene Instrumentation Spectroscopy Fluorescent Dyes Detection limit Microscopy Confocal biology Chemistry Optical Imaging Quinones equipment and supplies 021001 nanoscience & nanotechnology biology.organism_classification Atomic and Molecular Physics and Optics 0104 chemical sciences Spectrometry Fluorescence Proton NMR 0210 nano-technology Selectivity HeLa Cells |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 218:206-212 |
| ISSN: | 1386-1425 |
| Popis: | Hydrogen sulfide (H2S) is a kind of gaseous signal molecule in many physiological processes. In order to detect H2S, a novel “turn on” fluorescent probe 6,12-dihydroxyperylene-1,7-dione (DPD) was designed and synthesized. The probe DPD is fluorescence silence, while the addition of H2S induces an obvious green fluorescence with an obvious color change from dark blue to yellow-green. The probe shows excellent selectivity, fast response (2.5 min) and linear curve (0–90 μM) in wide effective pH range (4–10). Competition experiments are also revealed in corresponding studies and the detection limit is 3.6 μM. The response mechanism is proved to be the reduction of the probe by H2S, which is confirmed by 1H NMR. Furthermore, through the fluorescence turn-on signal toward H2S in Hela cells, probe DPD was successfully applied to monitor H2S in living Hela cells. |
| Databáze: | OpenAIRE |
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