Environmental DNA filtration techniques affect recovered biodiversity
Autor: | Erik Boström, Shannon H. C. Eagle, Mehrdad Hajibabaei, Markus Majaneva, Ola Håvard Diserud, Torbjørn Ekrem |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Preservation Biological Biodiversity lcsh:Medicine Fresh Water Dna recovery 010603 evolutionary biology 01 natural sciences Article law.invention 03 medical and health sciences chemistry.chemical_compound law Lysis buffer Animals Environmental DNA Cellulose lcsh:Science Filtration Multidisciplinary lcsh:R Lake ecosystem DNA Filter (signal processing) Pulp and paper industry 030104 developmental biology chemistry Environmental science lcsh:Q Environmental Monitoring |
Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Freshwater metazoan biodiversity assessment using environmental DNA (eDNA) captured on filters offers new opportunities for water quality management. Filtering of water in the field is a logistical advantage compared to transport of water to the nearest lab, and thus, appropriate filter preservation becomes crucial for maximum DNA recovery. Here, the effect of four different filter preservation strategies, two filter types, and pre-filtration were evaluated by measuring metazoan diversity and community composition, using eDNA collected from a river and a lake ecosystem. The filters were preserved cold on ice, in ethanol, in lysis buffer and dry in silica gel. Our results show that filters preserved either dry or in lysis buffer give the most consistent community composition. In addition, mixed cellulose ester filters yield more consistent community composition than polyethersulfone filters, while the effect of pre-filtration remained ambiguous. Our study facilitates development of guidelines for aquatic community-level eDNA biomonitoring, and we advocate filtering in the field, using mixed cellulose ester filters and preserving the filters either dry or in lysis buffer. © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
Databáze: | OpenAIRE |
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