Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products
| Autor: | Massimo Labra, Anna Sandionigi, Antonia Bruno, Maurizio Casiraghi, Giulia Agostinetto, Lorenzo Bernabovi, Jessica Frigerio |
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| Přispěvatelé: | Bruno, A, Sandionigi, A, Agostinetto, G, Bernabovi, L, Frigerio, J, Casiraghi, M, Labra, M |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Traceability Food Handling Sample (material) herbal products Food Contamination Molecular marker Biology Raw material food tracking 01 natural sciences DNA sequencing Article DNA metabarcoding processed food 03 medical and health sciences food matrices trnL Genetics DNA Barcoding Taxonomic Genetics (clinical) Plant Proteins Herbal product food matrice business.industry molecular markers High Throughput Sequencing High Throughput Sequencing 010401 analytical chemistry High-Throughput Nucleotide Sequencing Food composition data Sequence Analysis DNA Contamination Plants 0104 chemical sciences Biotechnology Food contaminant lcsh:Genetics 030104 developmental biology Food processing food contaminants business |
| Zdroj: | Genes Genes, Vol 10, Iss 3, p 248 (2019) Volume 10 Issue 3 |
| ISSN: | 2073-4425 |
| Popis: | One of the main goals of the quality control evaluation is to identify contaminants in raw material, or contamination after a food is processed and before it is placed on the market. During the treatment processes, contamination, both accidental and economically motivated, can generate incongruence between declared and real composition. In our study, we evaluated if DNA metabarcoding is a suitable tool for unveiling the composition of processed food, when it contains small trace amounts. We tested this method on different types of commercial plant products by using tnrL marker and we applied amplicon-based high-throughput sequencing techniques to identify plant components in different food products. Our results showed that DNA metabarcoding can be an effective approach for food traceability in different type of processed food. Indeed, the vast majority of our samples, we identified the species composition as the labels reported. Although some critical issues still exist, mostly deriving from the starting composition (i.e., variable complexity in taxa composition) of the sample itself and the different processing level (i.e., high or low DNA degradation), our data confirmed the potential of the DNA metabarcoding approach also in quantitative analyses for food composition quality control. |
| Databáze: | OpenAIRE |
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