Notch down‐regulation and inflammatory cytokines and RANKL overexpression involvement in peri‐implant mucositis and peri‐implantitis: A cross‐sectional study
| Autor: | Ana Djinic Krasavcevic, Jelena Carkic, Jelena Milasin, Sasha Jankovic, Zoran Aleksic, Iva Milinkovic, Marija Jezdic, Nadja Nikolic |
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| Rok vydání: | 2021 |
| Předmět: |
Mucositis
medicine.medical_specialty Peri-implant mucositis Osteolysis Notch signaling pathway Down-Regulation 03 medical and health sciences 0302 clinical medicine Osteoclast Internal medicine medicine Humans Notch 2 Notch 1 030304 developmental biology Dental Implants 0303 health sciences biology business.industry 030206 dentistry medicine.disease Peri-Implantitis 3. Good health Cross-Sectional Studies Endocrinology medicine.anatomical_structure RANKL biology.protein Cytokines Oral Surgery business |
| Zdroj: | Clinical Oral Implants Research. 32:1496-1505 |
| ISSN: | 1600-0501 0905-7161 |
| Popis: | OBJECTIVES Notch signaling pathway, known to influence bone resorption in several oral diseases, has not been analyzed in peri-implantitis yet. Therefore, the aims of the present study were to determine the levels of Notch cascade, bone remodeling mediators, and pro-inflammatory cytokines, in conjunction with clinical parameters, in subjects with peri-implant mucositis and peri-implantitis. MATERIAL AND METHODS Clinical parameters: peri-implant probing depth, bleeding on probing, suppuration on probing, and plaque index (PI) were recorded. Samples were collected from 130 participants, divided into peri-implantitis (PI), peri-implant mucositis (PM), and healthy implants (HI) group. Relative expression levels (REL) of Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-α, IL-17, IL-1β, IL-6, RANKL, and OPG mRNA were determined by reverse transcriptase-real-time polymerase chain reaction. Quantitation of Notch 1, Il-17, and IL-6 proteins was performed using ELISA assays. RESULTS All clinical parameters were significantly higher in PI compared to HI. Significant decrease of Notch 1, and higher REL of Hey 1, IL-1β, IL-6, and RANKL were found in PI compared to HI. PM showed significant increase of IL-1β REL in comparison with HI. In PI versus PM, significantly higher REL was found for Hey 1, TNF-α, IL-17, IL-1β, IL-6, and RANKL. Additionally, higher protein concentrations of IL-6 and IL-17 were detected in PI versus PM and versus HI group. CONCLUSION The combined effect of Notch 1 down-regulation and elevated expression of some key inflammation modulators might result in osteoclast activity increase and subsequent osteolysis in peri-implantitis. |
| Databáze: | OpenAIRE |
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