Two Novel Screening Methods for Selecting Monoclonal Antibodies Which Specifically Inhibit DNase I Enzyme Activity
| Autor: | Takashi Nakajima, Haruo Takeshita, Koichiro Kishi, Yoshimitsu Nakashima, Osamu Hosomi, Toshihiro Yasuda |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
chemistry.chemical_classification
Dose-Response Relationship Drug biology medicine.drug_class Immunology Antibodies Monoclonal General Medicine Monoclonal antibody Molecular biology Enzyme assay Enzyme Biochemistry chemistry biology.protein medicine Screening method Deoxyribonuclease I Humans Cell culture supernatant Selection Genetic Antibody Polyacrylamide gel electrophoresis |
| Zdroj: | Immunological Investigations. 27:145-152 |
| ISSN: | 1532-4311 0882-0139 |
| DOI: | 10.3109/08820139809089452 |
| Popis: | Two novel screening methods, single radial enzyme diffusion and the DNA-cast polyacrylamide gel electrophoresis, for selecting monoclonal antibodies which detect human deoxyribonuclease I (DNase I) enzyme activity are described. The former was adopted for initial screening to select potential objective antibodies from numerous hybridoma culture supernatants, because it was easy to perform and a powerful mass-screening tool. The latter was utilized for the subsequent precise selection of the antibodies in the supernatants selected after preliminary screening by the former, because it was clearly more accurate and sensitive, although the procedure was slightly more complicated. The consecutive use of these two methods resulted in the isolation of 25 anti-human DNase I antibodies, all of which specifically inhibited the activity of human DNase I. |
| Databáze: | OpenAIRE |
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