Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene
| Autor: | Duncan Craven, Martin Elphinstone, Lisa Wiley, Graham Robertson, Millar Whalley, Christopher W. Bell, Daria N. Love |
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| Rok vydání: | 1991 |
| Předmět: |
Genes
Viral Sequence analysis viruses Equine herpesvirus 1 Molecular Sequence Data Restriction Mapping medicine.disease_cause Homology (biology) Virus Gene product Open Reading Frames Viral Envelope Proteins Virology Sequence Homology Nucleic Acid Genetics medicine Simplexvirus Amino Acid Sequence Cysteine Molecular Biology Gene biology Base Sequence General Medicine biology.organism_classification Blotting Northern Molecular biology Open reading frame Herpes simplex virus Sequence Alignment Herpesvirus 1 Equid |
| Zdroj: | Virus genes. 5(4) |
| ISSN: | 0920-8569 |
| Popis: | A homologue of the herpes simplex virus (HSV) glycoprotein D gene has been identified in the genome of equine herpesvirus-1 (EHV-1, equine abortion virus). An open reading frame in the middle of the short unique (US) region is capable of encoding a polypeptide of 402 amino acids that has 26% and 20% of its residues matching pseudorabies virus (PRV) gp50 and HSV-1 gD, respectively. Despite this low level of similarity, the positional identity of six cysteine residues and certain motifs, and the location of the EHV-1 gene, clearly define the EHV-1 polypeptide as one of a family of “gD-like” proteins. Two transcripts of 3.3–3.6 kb and 5.4–5.9 kb were identified, consistent with coterminal mRNAs for the EHV-1 gD gene and the adjacent upstream gene, respectively. Partial sequencing of other regions in US also revealed EHV-1 homologues of HSV-1 gE and gI genes, and a possible equivalent gene to PRV gX. By analogy with the ability of HSV-1 gD and PRV gp50 to induce strong anti-viral immune responses, the EHV-1 gD gene product is expected to be an excellent candidate for development as a vaccine antigen. |
| Databáze: | OpenAIRE |
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