Superoxide Anion Production and Bioenergetic Profile in Young and Elderly Human Primary Myoblasts
Autor: | Giovanni Monaco, Ester Sara Di Filippo, Rosa Mancinelli, Simone Guarnieri, Rita La Rovere, Stefania Fulle, Geert Bultynck, Mariangela Marrone, Tiziana Pietrangelo |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Aging medicine.medical_specialty MITOCHONDRIAL DYSFUNCTION Bioenergetics Article Subject DISORDERS Oxidative phosphorylation Biochemistry SARCOPENIA 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine REGENERATION Internal medicine medicine Myocyte Glycolysis OXIDATIVE STRESS Science & Technology Myogenesis Chemistry Superoxide HUMAN SATELLITE CELLS Skeletal muscle General Medicine Cell Biology HUMAN SKELETAL-MUSCLE N-ACETYLCYSTEINE medicine.disease 030104 developmental biology medicine.anatomical_structure Endocrinology DIFFERENTIATION Sarcopenia Life Sciences & Biomedicine STEM-CELLS 030217 neurology & neurosurgery Research Article |
Zdroj: | Oxidative Medicine and Cellular Longevity |
ISSN: | 1942-0994 1942-0900 |
Popis: | Sarcopenia is the age-related loss of skeletal muscle mass, strength, and function. It is associated with regenerative difficulties by satellite cells, adult muscle stem cells, and alteration of oxidative management, mainly the increase in superoxide anions (O2•−). We aimed to investigate the relation between regenerative deficit in elderly and increase in O2•− production along with mitochondrial alterations. Myoblasts and myotubes from skeletal muscle of young and elderly healthy subjects (27.8 ± 6 and 72.4 ± 6.5 years old) were measured: (1) superoxide dismutase activity and protein content, (2) mitochondrial O2•− production levels, (3) O2•− production variability, and (4) mitochondrial bioenergetic profile. Compared to young myoblasts, elderly myoblasts displayed decreased SOD2 protein expression, elevated mitochondrial O2•− baseline levels, and decreased oxidative phosphorylation and glycolysis. Additionally, elderly versus young myotubes showed elevated mitochondrial O2•− levels when stressed with N-acetyl cysteine or high glucose and higher glycolysis despite showing comparable oxidative phosphorylation levels. Altogether, the elderly may have less metabolic plasticity due to the impaired mitochondrial function caused by O2•−. However, the increased energy demand related to the differentiation process appears to activate compensatory mechanisms for the partial mitochondrial dysfunction. |
Databáze: | OpenAIRE |
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