Chromatin tethering to the nuclear envelope by nuclear actin filaments: a novel role of the actin cytoskeleton in theXenopusblastula
| Autor: | Naoko Ura, Mari Iwabuchi, Haruka Oda, Natsuki Shirai, Keita Ohsumi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Nuclear Envelope Xenopus macromolecular substances Biology Xenopus laevis 03 medical and health sciences Genetics Animals Actin Ovum Cell-Free System Chromatin binding Cell Biology Blastula biology.organism_classification Actin cytoskeleton Actins Chromatin Cell biology Spindle apparatus Gastrulation Actin Cytoskeleton 030104 developmental biology |
| Zdroj: | Genes to Cells. 22:376-391 |
| ISSN: | 1356-9597 |
| DOI: | 10.1111/gtc.12483 |
| Popis: | TheXenopusoocyte is known to accumulate filamentous or F-actin in the nucleus, but it is currently unknown whether F-actin also accumulates in embryo nuclei. Using fluorescence-labeled actin reporters, we examined the actin distribution inXenopusembryonic cells and found that F-actin accumulates in nuclei during the blastula stage but not during the gastrula stage. To further investigate nuclear F-actin, we devised aXenopusegg extract that reproduces the formation of nuclei in which F-actin accumulates. Using this extract, we found that F-actin accumulates primarily at the sub-nuclear membranous region and is essential to maintain chromatin binding to the nuclear envelope in well-developed nuclei. We also provide evidence that nuclear F-actin increases the structural stability of nuclei and contributes to chromosome alignment on the mitotic spindle at the following M phase. These results suggest the physiological importance of nuclear F-actin accumulation in rapidly dividing, largeXenopusblastula cells. |
| Databáze: | OpenAIRE |
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