No genome-wide DNA methylation changes found associated with medium-term reduced graphene oxide exposure in human lung epithelial cells
| Autor: | Ricardo Santamaría, Mario F. Fraga, Ramón Torrecillas, Adolfo F. Valdes, Agustín F. Fernández, Raúl F. Pérez, Marta I. Sierra, Paula Morales-Sánchez, Anna Yunuen Soto Fernández, Clara Blanco, Juan Ramón Tejedor, Pablo Bousquets Muñoz |
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| Přispěvatelé: | Principado de Asturias, Asociación Española Contra el Cáncer, Fundación General CSIC, European Commission, Obra Social Cajastur, Pérez, Raúl F. [0000-0003-4336-9898], Tejedor, Juan Ramón [0000-0002-4061-9698], Morales, Paula [0000-0002-9563-668X], Torrecillas, Ramón [0000-0003-3856-0217], Fraga, Mario F. [0000-0001-8450-2603], Fernández, Agustín F. [0000-0002-3792-4085], Pérez, Raúl F., Tejedor, Juan Ramón, Morales, Paula, Torrecillas, Ramón, Fraga, Mario F., Fernández, Agustín F. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Biology Exposure Medium term Human lung Epigenome 03 medical and health sciences 0302 clinical medicine medicine Humans Molecular Biology Cells Cultured Nanomaterials DNA methylation Molecular biology Nanostructures 030104 developmental biology medicine.anatomical_structure Alveolar Epithelial Cells 030220 oncology & carcinogenesis Graphite Epigenetics Graphene Research Paper |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Epigenetics |
| ISSN: | 1559-2308 1559-2294 |
| DOI: | 10.1080/15592294.2019.1666650 |
| Popis: | The presence of nanomaterials in our everyday life is ever increasing, and so too are concerns about the possible health consequences of exposure to them. While evidence of their biological activity is growing, there is still scant knowledge of the epigenetic mechanisms that could be at play in these processes. Moreover, the great variability in the chemical and physical structures of these compounds handicaps the study of their possible health risks. Here we have synthesized reduced graphene oxide (rGO) through the thermal exfoliation/reduction of graphite oxide, and characterized the resulting material. We have then made use of Illumina's MethylationEPIC arrays and bisulphite pyrosequencing to analyse the genome-wide and global DNA methylation dynamics associated with the medium-term exposure of human lung epithelial cells to rGO at concentrations of 1 and 10 µg/mL. The results show no genome-wide or global DNA methylation changes associated with either condition. Our observations thus suggest that medium-term rGO exposure does not have significant effects on the DNA methylation patterns of human lung epithelial cells. This work has been financially supported by: The Plan Nacional de I+D+I co-funding FEDER (PI15/00892 and PI18/01527); the Government of the Principality of Asturias PCTI-Plan de Ciencia, Tecnología e Innovación de Asturias co-funding 2018-2022/FEDER (IDI/2018/146 and IDI/2018/121); AECC (PROYE18061FERN); FGCSIC (0348_CIE_6_E); IUOPA-ISPA-FINBA (The IUOPA is supported by the Obra Social Cajastur-Liberbank, Spain); A.F. Fernández is supported by a Miguel Servet II fellowship (contract CPII16/00007); R.F. Pérez is supported by ISPA-FINBA. |
| Databáze: | OpenAIRE |
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