HOP protein expression in the hippocampal dentate gyrus is acutely downregulated in a status epilepticus mouse model

Autor: Tomokatsu Hori, YA Alshebib, Taichi Kashiwagi
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RGL cell
Radial glia-like cell
medicine.medical_specialty
IHC
Immunohistochemistry
SVZ
subventricular zone
Neurosciences. Biological psychiatry. Neuropsychiatry
Status epilepticus
BrdU
5-Bromo-2′-deoxyuridine
Neurocardiology
Biology
Hippocampal formation
Epileptogenesis
Hop (networking)
DCX
Doublecortin
Internal medicine
HDG
Hippocampal Dentate Gyrus
Status Epileptics
medicine
Hippocampus (mythology)
Prox1
Prospero Homeobox 1
Neuroinflammation
GFP
green fluorescent protein
HOP
Homeodomain Only Protein
HF
Hippocampus Formation
HOP
General Neuroscience
Dentate gyrus
Hippocampal Formation
SGZ
subgranular zone
BLBP
Brain lipid-binding protein
EGFP
enhanced green fluorescent protein
S100β
S100 calcium-binding protein B
Endocrinology
Gliosis
Seizure-induced neuroinflammation
Epileptogenicity
Homeodomain-Only Protein
GCL
granule cell layer
medicine.symptom
Ctrl
control tissue
NSC
Neural stem cells
SE
Status Epilepticus
GFAP
Glial fibrillary acidic protein
Research Paper
RC321-571
Zdroj: IBRO Neuroscience Reports, Vol 11, Iss, Pp 183-193 (2021)
IBRO Neuroscience Reports
ISSN: 2667-2421
Popis: Status epilepticus (SE) is a neurological emergency, and delayed management can lead to higher morbidity and mortality. It is thought that prolonged seizures stimulate stem cells in the hippocampus and that epileptogenesis may arise from aberrant connections formed by newly born cells, while others have suggested that the acute neuroinflammation and gliosis often seen in epileptic hippocampi contribute to hyperexcitability and epilepsy development. Previous studies have identified the expression of homeodomain-only protein (HOP) in the hippocampal dentate gyrus (HDG) and the heart. HOP was found to be a regulator of cell proliferation and differentiation during heart development, while it maintains the ‘heart conduction system’ in adulthood. However, little is known about HOP function in the adult HDG, particularly in the SE setting. Here, a HOP immunohistochemical profile in an SE mouse model was established. A total of 24 adult mice were analyzed 3–10 days following the SE episode, the ‘acute phase’. Our findings demonstrate a significant downregulation of HOP and BLBP protein expression in the SE group following SE episodes, while HOP/Ki67 coexpression did not remarkably differ. Furthermore, coexpression of HOP/S100β and HOP/Prox1 was not observed, although we noticed insignificant HOP/DCX coexpression level. The findings of this study show no compelling evidence of proliferation, and newly added neurons were not identified during the acute phase following SE, although HOP protein expression was significantly decreased in the HDG. Similar to its counterpart in the adult heart, this suggests that HOP seems to play a key role in regulating signal conduction in adult hippocampus. Moreover, acute changes in HOP expression following SE could be part of an inflammatory response that could subsequently influence epileptogenicity.
Highlights • Homeodomain-only protein (HOP) is exclusively expressed in adult hippocampal dentate gyrus. • In adult HDG, HOP-expressing cells could represent a unique terminally-differentiated, highly specialized glial subtype. • . A single episode of status epilepticus (SE) does not affect cell proliferation nor differentiation in adult HDG. • HOP protein is downregulated following SE and could influence epileptogenicity. • Similar to its function in adult heart, HOP seems to play critical role in regulating adult ‘hippocampus conduction system’. • HOP protein could represent a molecular target for acute management of SE to minimize its cumulative effects.
Databáze: OpenAIRE
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