Analysis of historical negative control group data from the rat in vivo micronucleus assay
| Autor: | U. Hemmann, Jim Saul, James Whitwell, B.M. van der Leede, Stephen D. Dertinger, P. Escobar, Andreas Zeller, Julia Kenny, Robert Smith, David P. Lovell, R. Fiedler, Laura Custer, Mick D. Fellows |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Quality Control 0301 basic medicine Laboratory Proficiency Testing Health Toxicology and Mutagenesis Negative control 010501 environmental sciences Biology 01 natural sciences Rats Sprague-Dawley Andrology 03 medical and health sciences In vivo Genetics Animals Rats Wistar Micronuclei Chromosome-Defective 0105 earth and related environmental sciences Observer Variation Micronucleus Tests Reproducibility of Results Mean frequency Rats 030104 developmental biology Micronucleus test Female |
| ISSN: | 1383-5718 |
| Popis: | A database of micronuclei counts for historical negative control data from rat in vivo micronuclei tests performed in 10 different laboratories was established. Data were available from over 4000 negative control rats from 10 laboratories. The mean frequency of micronucleated cells (MN)/1000 cells ranged from 0.44 to 2.22, a 5-fold range. Overall there were no major sex or strain differences in frequency, although there were some small but statistically significant differences within laboratories. There was appreciable variability between experiments compared with variability within experiments in some laboratories. No specific factor was identified which could explain this variability although it was noted that many different vehicles were used in the experiments. It is hoped that these data will help laboratories beginning studies with the rat micronucleus assay and those involved in the assessment of micronucleus assay results. |
| Databáze: | OpenAIRE |
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