Performance of a New Microfluidic Dengue NS1 Immuno-magnetic Agglutination Assay for the Rapid Diagnosis of Dengue Infection in Adults
| Autor: | Ekkarat Wongsawat, Kanigar Niwattayakul, Nasikarn Angkasekwinai, Eakkawit Yamasmith, Susan Assanasaen, Yupin Suputtamongkol, Chunya Puttikhunt, Panisadee Avirutnan, Tanapan Prommool, Saowaluk Silpasakorn |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Adolescent viruses Ns1 antigen Viral Nonstructural Proteins Sensitivity and Specificity law.invention Dengue fever Dengue Young Adult law Virology Agglutination Tests Lab-On-A-Chip Devices parasitic diseases Microchip Analytical Procedures Medicine Humans Serologic Tests Antigens Viral Polymerase chain reaction Aged Glycoproteins Rapid diagnostic test business.industry virus diseases Articles biochemical phenomena metabolism and nutrition Dengue Virus Middle Aged medicine.disease Infectious Diseases Agglutination assay Magnets Parasitology Female Detection rate business |
| Zdroj: | Am J Trop Med Hyg |
| ISSN: | 1476-1645 |
| Popis: | Dengue (DENV) infections are a public health concern worldwide and thus early diagnosis is important to ensure appropriate clinical management. The rapid diagnostic test (RDT) targets nonstructural protein 1 (NS1) detection and is the main tool used for diagnostic purpose. In this study, we evaluated the performance of a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic agglutination assay or IMA (ViroTrack Dengue Acute, BluSense Diagnostics, Copenhagen, Denmark). We studied 233 subjects confirmed to have DENV infection (by a real-time reverse transcriptase polymerase chain reaction) and 200 control samples were taken from patients with confirmed diagnoses of other febrile illnesses, in Thailand. Samples were tested using the NS1 antigen (Ag) detection methods: in-house NS1 Ag ELISA (ELISA), SD BIOLINE Dengue NS1 Ag RDT (ICT), and ViroTrack Dengue Acute (IMA). Sensitivities of these tests were 86.3%, 78.9%, and 85.5%, respectively. All tests showed high specificity (100%, 99%, and 97% for ELISA, ICT, and IMA, respectively). The sensitivities of both RDTs were affected by the low sensitivity to DENV-2 and DENV-4. NS1 Ag was detected in every patient on day 1 and day 2 after onset of illness by ELISA and IMA with a decline in detection rates over time after day 6 of illness. NS1 detection rate using ICT decreased from 100% on day 1 of illness to 98.6% on day 2 after onset of illness. By day 6, the detection rate was 45.9%. Thus, IMA performed better than ICT for early and rapid diagnosis of DENV infections in endemic countries. |
| Databáze: | OpenAIRE |
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