In vivo effects of curcumin and deferoxamine in experimental endometriosis

Autor: Gulay Seren, Enis Uluçam, Ziya Cukur, Umit A. Kayisli, Ali Yilmaz, Gulnur Kizilay, Yesim Hulya Uz
Rok vydání: 2017
Předmět:
medicine.medical_specialty
Curcumin
Iron
Ovariectomy
Endometriosis
H&E stain
Uterus
Siderophores
Medicine (miscellaneous)
Deferoxamine
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Peritoneum
In vivo
Proliferating Cell Nuclear Antigen
Internal medicine
Biopsy
Internal Medicine
medicine
Animals
Humans
Pharmacology (medical)
Rats
Wistar
Genetics (clinical)
Cell Proliferation
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
business.industry
Anti-Inflammatory Agents
Non-Steroidal
Keratin-7
Drug Synergism
medicine.disease
Immunohistochemistry
Disease Models
Animal
medicine.anatomical_structure
Endocrinology
chemistry
030220 oncology & carcinogenesis
Reviews and References (medical)
Female
business
medicine.drug
Zdroj: Advances in Clinical and Experimental Medicine. 26:207-213
ISSN: 1899-5276
DOI: 10.17219/acem/31186
Popis: Background Endometriosis is one of the most common chronic gynecological diseases. Objectives The aim of the study was to examine the effects of curcumin and/or deferoxamine on cell proliferation in a rat model of endometriosis. Material and methods Thirty female 12-week-old albino Wistar rats, weighing 200-250 g, were used in this study. All the rats underwent ovariectomy and 0.1-mg β-estradiol 17-valerate pellets were placed intraperitoneally. An experimental model of endometriosis was created in all the animals. To create the experimental model, an approximately 1-cm long section of the uterus was taken, primarily from the right horn of the uterus. Autologous fragments were then placed between the peritoneum and muscle. The animals were divided into 3 groups: Group A, treated only with the vehicle used for curcumin and deferoxamine; group B, treated with curcumin (100 mg/kg body weight); and group C, treated with deferoxamine + curcumin (100 mg/kg body weight). After biopsy samples were obtained, the sections were stained with hematoxylin and eosin. Immunostaining for cytokeratin-7 and proliferating cell nuclear antigen (PCNA) was performed. Blood iron levels were measured using a Perkin Elmer AAnalyst 800 Atomic Absorption Spectrophotometer. Results The endometrial implant size increased in Group A, but treatment with curcumin (p = 0.01) and deferoxamine + curcumin (p = 0.007) reduced the implant size. In ectopic endometrial epithelial cells, there were significant decreases in PCNA immunoreactivity between groups A and B (p = 0.044) and between groups A and C (p = 0.033). Conclusions Treatment with curcumin alone and/or in combination with deferoxamine contributed to a reduction in implant size and cell proliferation in a rat endometriosis model. Iron-chelating agents may act in the same manner when used in women with endometriosis; however, further studies from different perspectives are still needed.
Databáze: OpenAIRE
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