Capacity of a hydroxyapatite–lysozyme combination against Streptococcus mutans for the treatment of dentinal caries
| Autor: | Sérgio Luiz Pinheiro, Nathany Nunes da Rocha, Mariane de Lourdes Hernandes Martins Peres |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Molar Materials science Dentistry Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound stomatognathic system Dentin medicine Statistical analysis General Dentistry 030102 biochemistry & molecular biology biology business.industry Antimicrobial biology.organism_classification In Vitro Study enzyme stomatognathic diseases medicine.anatomical_structure chemistry Dental caries Sodium laureth sulfate Lysozyme business After treatment |
| Zdroj: | Journal of Conservative Dentistry : JCD |
| ISSN: | 0972-0707 |
| DOI: | 10.4103/0972-0707.190026 |
| Popis: | Background: One current strategy for the treatment of carious lesions is the use of biomaterials with antimicrobial activity. Aims: The aim of this study was to evaluate a combination of hydroxyapatite and lysozyme for the treatment of dentinal caries by measuring Streptococcus mutans counts before carious tissue sealing, and 24 h, 1 month, and 6 months after treatment. Materials and Methods: Forty permanent third molars were selected, and flat dentin surfaces were prepared. The teeth were exposed to a cariogenic challenge with S. mutans. After challenge, the dentinal caries were collected from five specimens. The remaining specimens were treated with a mixture of hydroxyapatite and lysozyme in sodium laureth sulfate and sealed with composite resin. S. mutans counts were obtained 24 h, 1 month, and 6 months after sealing. Statistical Analysis: The results were evaluated by descriptive statistics and Wilcoxon signed-rank test. Results: a significant reduction in S. mutans (CFU/mL) was observed in dentinal lesions 1 month after treatment with hydroxyapatite/lysozyme in sodium laureth sulfate (P = 0.0254). Comparison of S. mutans counts obtained 24 h, 1 month, and 6 months after treatment revealed reductions only at the 1-month time point (P = 0.0318). Conclusions: the combination of hydroxyapatite and lysozyme may be an alternative for reducing the S. mutans burden in dentinal caries. |
| Databáze: | OpenAIRE |
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