Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor
| Autor: | Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen, Qi-Yuan Guan, Cong-Hui Xu, Yin-Zhu Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
endocrine system
Analytical chemistry 02 engineering and technology Biosensing Techniques Selective excitation 010402 general chemistry 01 natural sciences Analytical Chemistry Coordination Complexes Organometallic Compounds Electrochemiluminescence Humans Electrodes Human blood Propylamines Chemistry Electrochemical Techniques Prostate-Specific Antigen 021001 nanoscience & nanotechnology 0104 chemical sciences Anode Concentration dependent embryonic structures Electrode Luminescent Measurements 0210 nano-technology Biosensor |
| Zdroj: | Analytical chemistry. 89(15) |
| ISSN: | 1520-6882 |
| Popis: | We report a multicolor ECL device based on closed bipolar electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)3]2+ and [Ir(ppy)3] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green–yellow–red ECL emission changing. As the first multicolor ECL dev... |
| Databáze: | OpenAIRE |
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