Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)

Autor: Chris Detter, Scott E. Baker, Cheryl R. Kuske, Nina Thayer, Randy M. Berka, Michael Ward, Igor V. Grigoriev, Conrad L. Schoch, Edward M. Rubin, Daniel S. Rokhsar, Isaac Ho, Alfredo Lopez de Leon, Susan Lucas, Barbara Robbertse, Paul G. Richardson, Mikko Arvas, Cliff Han, Monica Misra, Melissa Jackson, Beth Nelson, Luis F. Larrondo, Bernard Henrissat, Astrid Terry, Diego Martinez, Dan Cullen, Jarod Chapman, Etienne Danchin, Jian Yao, Gary Xie, Jon K. Magnuson, Thomas Brettin, Mary Anne Nelson, Nigel Dunn-Coleman, Asaf Salamov, David Bruce, Ann Westerholm-Parvinen, Nicholas H. Putnam, Monika Schmoll, Olga Chertkov, Pedro M. Coutinho, Sandy Merino, Paul Harris, Christian P. Kubicek, Markku Saloheimo, Ravi D. Barabote
Přispěvatelé: Los Alamos National Laboratory (LANL), Novozymes, Inc., Partenaires INRAE, Architecture et fonction des Macromolécules Biologiques - UMR 6098 (AFMB), Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), VTT Technical Research Centre of Finland (VTT), Pacific Northwest National Laboratory (PNNL), Joint Genome Institute (JGI), United States Department of Agriculture (USDA), Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), Vienna University of Technology, Pontificia Universidad Católica de Chile (UC), Department of Botany and Plant Pathology, Oregon State University (OSU), Genencor International, Department of Biology [New Mexico], The University of New Mexico [Albuquerque], AlerGenetiCa SL, Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Zdroj: Nature Biotechnology
Nature Biotechnology, 2008, 26, pp.553-560. ⟨10.1038/nbt1403⟩
Nature Biotechnology, Nature Publishing Group, 2008, 26, pp.553-560. ⟨10.1038/nbt1403⟩
Martinez, D, Berka, R M, Henrissat, B, Saloheimo, M, Arvas, M, Baker, S E, Chapman, J, Chertkov, O, Coutinho, P M, Cullen, D, Danchin, E G J, Grigoriev, I V, Harris, P, Jackson, M, Kubicek, C P, Han, C S, Ho, I, Larrondo, L F, de Leon, A L, Magnuson, J K, Merino, S, Misra, M, Nelson, B, Putnam, N, Robbertse, B, Salamov, A A, Schmoll, M, Terry, A, Thayer, N, Westerholm-Parvinen, A, Schoch, C L, Yao, J, Barabote, R, Barbote, R, Nelson, M A, Detter, C, Bruce, D, Kuske, C R, Xie, G, Richardson, P, Rokhsar, D S, Lucas, S M, Rubin, E M, Dunn-Coleman, N, Ward, M & Brettin, T S 2008, ' Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) ', Nature Biotechnology, vol. 26, no. 5, pp. 553-560 . https://doi.org/10.1038/nbt1403
ISSN: 1087-0156
DOI: 10.1038/nbt1403⟩
Popis: International audience; Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assembled 89 scaffolds (sets of ordered and oriented contigs) to generate 34 Mbp of nearly contiguous T. reesei genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering the industrial utility and effectiveness of the carbohydrate-active enzymes of T. reesei, its genome encodes fewer cellulases and hemicellulases than any other sequenced fungus able to hydrolyze plant cell wall polysaccharides. Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters that lie between regions of synteny with other Sordariomycetes. Numerous genes encoding biosynthetic pathways for secondary metabolites may promote survival of T. reesei in its competitive soil habitat, but genome analysis provided little mechanistic insight into its extraordinary capacity for protein secretion. Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production.
Databáze: OpenAIRE
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