Distinct subpopulations of enteric neuronal progenitors defined by time of development, sympathoadrenal lineage markers and Mash-1-dependence
| Autor: | Virginia M. Tennyson, Tuan Duc Pham, E. Blaugrund, Michael D. Gershon, L. Lo, D.J. Anderson, Lukas Sommer |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Sympathetic Nervous System Time Factors Molecular Sequence Data In Vitro Techniques Calcitonin gene-related peptide Biology Serotonergic Enteric Nervous System Rats Sprague-Dawley Mice Pregnancy Internal medicine Basic Helix-Loop-Helix Transcription Factors medicine Animals Molecular Biology DNA Primers Progenitor Neurons Regulation of gene expression Base Sequence Tyrosine hydroxylase Stem Cells Lineage markers Gene Expression Regulation Developmental Antigens Differentiation Immunohistochemistry Mice Mutant Strains Rats Cell biology DNA-Binding Proteins Mice Inbred C57BL Endocrinology nervous system Calcitonin Mutation Female MASH-1 Transcription Factors Developmental Biology |
| Zdroj: | Development. 122:309-320 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.122.1.309 |
| Popis: | Enteric and sympathetic neurons have previously been proposed to be lineally related. We present independent lines of evidence that suggest that enteric neurons arise from at least two lineages, only one of which expresses markers in common with sympathoadrenal cells. In the rat, sympathoadrenal markers are expressed, in the same order as in sympathetic neurons, by a subset of enteric neuronal precursors, which also transiently express tyrosine hydroxylase. If this precursor pool is eliminated in vitro by complement-mediated lysis, enteric neurons continue to develop; however, none of these are serotonergic. In the mouse, the Mash-1−/− mutation, which eliminates sympathetic neurons, also prevents the development of enteric serotonergic neurons. Other enteric neuronal populations, however, including those that contain calcitonin gene related peptide are present. Enteric tyrosine hydroxylasecontaining cells co-express Mash-1 and are eliminated by the Mash-1−/− mutation, consistent with the idea that in the mouse, as in the rat, these precursors generate serotonergic neurons. Serotonergic neurons are generated early in development, while calcitonin gene related peptide-containing enteric neurons are generated much later. These data suggest that enteric neurons are derived from at least two progenitor lineages. One transiently expresses sympathoadrenal markers, is Mash-1-dependent, and generates early-born enteric neurons, some of which are serotonergic. The other is Mash-1-independent, does not express sympathoadrenal markers, and generates late-born enteric neurons, some of which contain calcitonin gene related peptide. |
| Databáze: | OpenAIRE |
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