The structure of FCPb, a light-harvesting complex in the diatom Cyclotella meneghiniana

Autor: Claudia Büchel, Anja Röding, Egbert J. Boekema
Přispěvatelé: Electron Microscopy
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Photosystem II
Stereochemistry
OLIGOMERIC STATES
Light-Harvesting Protein Complexes
Oligomeric state
Trimer
Plant Science
01 natural sciences
Biochemistry
SUPERCOMPLEX
CHLAMYDOMONAS-REINHARDTII
Single particle analysis
Light-harvesting complex
03 medical and health sciences
chemistry.chemical_compound
PHOTOSYSTEM-II
Electron microscopy
Fucoxanthin
THYLAKOID MEMBRANES
Phaeodactylum tricornutum
FLUORESCENCE
PHAEODACTYLUM-TRICORNUTUM
Diatoms
DIATOXANTHIN
SPECTROSCOPY
biology
Diatoxanthin
Cell Biology
General Medicine
biology.organism_classification
Fucoxanthin chlorophyll protein
030104 developmental biology
Diatom
chemistry
Chromatography
Gel
Chlorophyll Binding Proteins
Protein Multimerization
FUCOXANTHIN-CHLOROPHYLL-PROTEINS
010606 plant biology & botany
Zdroj: Photosynthesis Research, 135(1-3), 203-211. SPRINGER
ISSN: 0166-8595
Popis: Diatoms possess fucoxanthin chlorophyll proteins (FCP) as light-harvesting systems. These membrane intrinsic proteins bind fucoxanthin as major carotenoid and Chl c as accessory chlorophyll. The relatively high sequence homology to higher plant light-harvesting complex II gave rise to the assumption of a similar overall structure. From centric diatoms like Cyclotella meneghiniana, however, two major FCP complexes can be isolated. FCPa, composed of Fcp2 and Fcp6 subunits, was demonstrated to be trimeric, whereas FCPb, known to contain Fcp5 polypeptides, is of higher oligomeric state. No molecular structure of either complex is available so far. Here we used electron microscopy and single particle analysis to elucidate the overall architecture of FCPb. The complexes are built from trimers as basic unit, assembling into nonameric moieties. The trimer itself is smaller, i.e. more compact than LHCII, but the main structural features are conserved.
Databáze: OpenAIRE
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