Towards embedding Caco-2 model of gut interface in a microfluidic device to enable multi-organ models for systems biology

Autor:	Irina Tsypina, Ancha Baranova, E N Knyazev, D. V. Maltseva, S A Shilin, Andrey A. Poloznikov, D. A. Sakharov, Alexander G. Tonevitsky, Sergey Nikulin
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	2D layer Systems biology 0206 medical engineering Microfluidics 02 engineering and technology Caco-2 cell lines Structural Biology Lab-On-A-Chip Devices microRNA Cell Adhesion Humans Colorectal adenocarcinoma On-the-chip models Molecular Biology lcsh:QH301-705.5 Cell adhesion molecule Chemistry Applied Mathematics Research Microcirculation Systems Biology Cell Membrane Cell Differentiation Multi organ Computer Science Applications Cell biology Intestines Microfluidic chip lcsh:Biology (General) Caco-2 Modeling and Simulation Caco-2 Cells 020602 bioinformatics
Zdroj:	BMC Systems Biology, Vol 13, Iss S1, Pp 3-11 (2019) BMC Systems Biology
ISSN:	1752-0509
DOI:	10.1186/s12918-019-0686-y
Popis:	Background A cancer cell line originating from human epithelial colorectal adenocarcinoma (Caco-2 cells) serves as a high capacity model for a preclinical screening of drugs. Recent need for incorporating barrier tissue into multi-organ chips calls for inclusion of Caco-2 cells into microperfused environment. Results This article describes a series of systems biology insights obtained from comparing Caco-2 models cells grown as conventional 2D layer and in a microfluidic chip. When basic electrical parameters of Caco-2 monolayers were evaluated using impedance spectrometry and MTT assays, no differences were noted. On the other hand, the microarray profiling of mRNAs and miRNAs revealed that grows on a microfluidic chip leads to the change in the production of specific miRNA, which regulate a set of genes for cell adhesion molecules (CAMs), and provide for more complete differentiation of Caco-2 monolayer. Moreover, the sets of miRNAs secreted at the apical surface of Caco-2 monolayers grown in conventional 2D culture and in microfluidic device differ. Conclusions When integrated into a multi-tissue platform, Caco-2 cells may aid in generating insights into complex pathophysiological processes, not possible to dissect in conventional cultures.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b1c6ea84cf13b8e0b8d07acfdffda5 http://link.springer.com/article/10.1186/s12918-019-0686-y Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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