Rapid screening of marine bacterial symbionts using MALDI-TOF MS
| Autor: | Maria Cecília de Souza Nóbrega, Livia Vidal, Hannah K Mattsson, Diogo A. Tschoeke, Aline R. P. Gonçalves, Fabiano L. Thompson, Cristiane C. Thompson, Verônica Viana Vieira, Tainá Venas, Gustavo P R Azevedo, Gizele D. Garcia, Raphael V P Silva |
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| Rok vydání: | 2020 |
| Předmět: |
Ruegeria
Marine Biology Mass spectrometry Biochemistry Microbiology 03 medical and health sciences RNA Ribosomal 16S Genetics Environmental Microbiology Seawater Alteromonas Symbiosis Molecular Biology 030304 developmental biology Vibrio 0303 health sciences biology 030306 microbiology Chemistry General Medicine biology.organism_classification 16S ribosomal RNA Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Pseudovibrio Bacteria |
| Zdroj: | Archives of microbiology. 202(8) |
| ISSN: | 1432-072X |
| Popis: | Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups’ identification. |
| Databáze: | OpenAIRE |
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