Expression of the C4 Me1 Gene from Flaveria bidentis Requires an Interaction between 5[prime] and 3[prime] Sequences

Autor:	Brian P. Surin, Jerry Stuart Marshall, Julie A. Chitty, John D. Stubbs, William C. Taylor
Rok vydání:	1997
Předmět:	Flaveria bidentis Genetics chemistry.chemical_classification Reporter gene fungi Cell Biology Plant Science Biology Vascular bundle Prime (order theory) Cell biology Enzyme chemistry Cell separation Gene C4 photosynthesis Research Article
Zdroj:	The Plant Cell. :1515-1525
ISSN:	1532-298X 1040-4651
Popis:	The efficient functioning of C4 photosynthesis requires the strict compartmentation of a suite of enzymes in either mesophyll or bundle sheath cells. To determine the mechanism controlling bundle sheath cell-specific expression of the NADP-malic enzyme, we made a set of chimeric constructs using the 5[prime] and 3[prime] regions of the Flaveria bidentis Me1 gene fused to the [beta]-glucuronidase gusA reporter gene. The pattern of GUS activity in stably transformed F. bidentis plants was analyzed by histochemical and cell separation techniques. We conclude that the 5[prime] region of Me1 determines bundle sheath specificity, whereas the 3[prime] region contains an apparent enhancer-like element that confers high-level expression in leaves. The interaction of 5[prime] and 3[prime] sequences was dependent on factors that are present in the C4 plant but not found in tobacco.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b0eedb76abea6935025e300e00aaba https://doi.org/10.1105/tpc.9.9.1515 Zobrazit plný text záznamu